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We have defined conditions for generating large numbers of dendritic cells (DC) in marrow cultures from 10-12-week-old ACI or WF rats. The combination of granulocyte-macrophage colony-stimulating factor (GM-CSF) and TNF-alpha, known to induce DC from human CD34+ progenitors, was not effective with rat. In contrast, GM-CSF plus IL-4 generated DC in high yield, corresponding to 30-40% of the initial number of plated marrow cells. The DC proliferated in distinctive aggregates, in which most cells had an immature phenotype marked by undetectable surface B7 and high levels of MHC class II products within intracellular lysosomes. When dislodged and dispersed, the aggregates gave rise to mature stellate DC with abundant surface MHC class II and B7, sparse MHC class II- lysosomes, and strong T cell-stimulating capacity. Therefore, rat marrow progenitors can generate large numbers of immature DC, with abundant intracellular MHC class II compartments, and potent, stimulatory, mature DC.
M Talmor, and
A Mirza, and
S Turley, and
I Mellman, and
L A Hoffman, and
R M Steinman
December 1992,
The Journal of experimental medicine,
M Talmor, and
A Mirza, and
S Turley, and
I Mellman, and
L A Hoffman, and
R M Steinman
January 2001,
Methods in molecular medicine,
M Talmor, and
A Mirza, and
S Turley, and
I Mellman, and
L A Hoffman, and
R M Steinman
November 2000,
Blood,
M Talmor, and
A Mirza, and
S Turley, and
I Mellman, and
L A Hoffman, and
R M Steinman
February 2023,
Scientific reports,
M Talmor, and
A Mirza, and
S Turley, and
I Mellman, and
L A Hoffman, and
R M Steinman
November 2006,
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
M Talmor, and
A Mirza, and
S Turley, and
I Mellman, and
L A Hoffman, and
R M Steinman
February 1999,
Journal of immunological methods,
M Talmor, and
A Mirza, and
S Turley, and
I Mellman, and
L A Hoffman, and
R M Steinman
July 2011,
Nanoscale research letters,
M Talmor, and
A Mirza, and
S Turley, and
I Mellman, and
L A Hoffman, and
R M Steinman
April 2003,
American journal of clinical pathology,
M Talmor, and
A Mirza, and
S Turley, and
I Mellman, and
L A Hoffman, and
R M Steinman
April 2013,
Experimental and molecular pathology,
M Talmor, and
A Mirza, and
S Turley, and
I Mellman, and
L A Hoffman, and
R M Steinman
April 1995,
Transplantation proceedings,
