Biomaterial Database

Prolonged in vitro culture modifies the surface lipid composition of murine melanoma cell lines. 1997

S Moretti, and G Montorfano, and S Rapelli, and B Berra

Institute of General Physiology and Biochemistry, School of Pharmacy, University of Milan, Italy.

Lipid composition of two murine melanoma cell variants (B16, without malignant properties and B16-F10, with high metastatic activity), has been examined at different stages of growth. The aim of the work was to identify cell surface modifications due to the time length of in vitro culture, that could be one variable to consider when metastatic potential is studied. Some of the analyzed parameters (ganglioside- and glycoprotein-bound neuraminic acid, cholesterol, neutral glycolipids, phospholipids, triacylglycerols) undergo statistically significant variations at the various passages in B16-F10 line. Fatty acids composition of the phospholipidic fraction was changed only at the last observed passage (100) in B16 line. No one of the examined parameters justifies the ability of B16-F10 cells to invade distant districts and to originate new tumors. Probably detailed lipid analysis on cellular subfractions, as already performed in this study on total lipid extract of the whole cell, could be a valuable tool to identify differences related with metastatic potential.

UI	MeSH Term	Description	Entries
D008546	Melanoma, Experimental	Experimentally induced tumor that produces MELANIN in animals to provide a model for studying human MELANOMA.	B16 Melanoma,Melanoma, B16,Melanoma, Cloudman S91,Melanoma, Harding-Passey,Experimental Melanoma,Experimental Melanomas,Harding Passey Melanoma,Melanomas, Experimental,B16 Melanomas,Cloudman S91 Melanoma,Harding-Passey Melanoma,Melanoma, Harding Passey,Melanomas, B16,S91 Melanoma, Cloudman
D008563	Membrane Lipids	Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.	Cell Membrane Lipid,Cell Membrane Lipids,Membrane Lipid,Lipid, Cell Membrane,Lipid, Membrane,Lipids, Cell Membrane,Lipids, Membrane,Membrane Lipid, Cell,Membrane Lipids, Cell
D009362	Neoplasm Metastasis	The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.	Metastase,Metastasis,Metastases, Neoplasm,Metastasis, Neoplasm,Neoplasm Metastases,Metastases
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014407	Tumor Cells, Cultured	Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.	Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus