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The biosynthesis of carnitine from lysine and methionine involves five enzymatic reactions. Gamma-butyrobetaine hydroxylase (BBH; EC 1.14.11.1) is the last enzyme of this pathway. It catalyzes the reaction of hydroxylation of gamma-butyrobetaine to carnitine. This enzyme had never been purified to homogeneity from rat tissue. This paper describes the purification and characterization of the rat liver BBH. This protein has been purified some 413 fold by ion exchange, affinity and gel-filtration chromatographies and appears as a dimere of 43,000 Daltons subunits by PAGE. The affinity chromatography column used in the purification process utilizes 3-(2,2,2-trimethylhydrazinium)propionate (THP), a BBH inhibitor, as the ligand. Polyclonal antibodies were raised against the liver enzyme. They were able to precipitate BBH activity in either a crude liver extract or a purified fraction of the enzyme. Furthermore, it crossreacts with a 43 kDa protein in the liver. No evidence for extra hepatic enzyme was found.
S Galland, and
F Le Borgne, and
D Guyonnet, and
P Clouet, and
J Demarquoy
January 1999,
Advances in experimental medicine and biology,
S Galland, and
F Le Borgne, and
D Guyonnet, and
P Clouet, and
J Demarquoy
December 1981,
Archives of biochemistry and biophysics,
S Galland, and
F Le Borgne, and
D Guyonnet, and
P Clouet, and
J Demarquoy
August 1970,
The Journal of biological chemistry,
S Galland, and
F Le Borgne, and
D Guyonnet, and
P Clouet, and
J Demarquoy
May 1993,
European journal of biochemistry,
S Galland, and
F Le Borgne, and
D Guyonnet, and
P Clouet, and
J Demarquoy
August 1987,
Biology of reproduction,
S Galland, and
F Le Borgne, and
D Guyonnet, and
P Clouet, and
J Demarquoy
May 1977,
Biochemistry,
S Galland, and
F Le Borgne, and
D Guyonnet, and
P Clouet, and
J Demarquoy
October 1982,
Scandinavian journal of clinical and laboratory investigation,
S Galland, and
F Le Borgne, and
D Guyonnet, and
P Clouet, and
J Demarquoy
August 2010,
Biochemical and biophysical research communications,
S Galland, and
F Le Borgne, and
D Guyonnet, and
P Clouet, and
J Demarquoy
February 1979,
FEBS letters,
S Galland, and
F Le Borgne, and
D Guyonnet, and
P Clouet, and
J Demarquoy
May 1967,
Biochemistry,
