Biomaterial Database

Determination of 4-hexylresorcinol in crab meat. 1998

A W Smallwood, and T L Ranieri, and R D Satzger

U.S. Food and Drug Administration, National Forensic Chemistry Center, Cincinnati, OH 45202, USA.

A method is described for determining 4-hexylresorcinol in crab meat. 4-Hexylresorcinol is used to prevent melanosis in shrimp, and the same use has been proposed for crab meat. Because 4-hexylresorcinol may be added illegally to crab meat as a preservative, consumer protection requires that residues of the compound be monitored in crab meat. 4-Hexylresorcinol is extracted from crab meat with acetonitrile. After dilution with water, the extract is passed through a C18 solid-phase extraction column and 4-hexylresorcinol is eluted from the column with ethanol. The compound is determined by reversed-phase liquid chromatography with diode array detection at 206 nm. Limit of quantitation is 1.0 microgram/g. Mean recovery in the range 1-20 micrograms/g is 89%, with a relative standard deviation of 6.3.

UI	MeSH Term	Description	Entries
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008460	Meat	The edible portions of any animal used for food including cattle, swine, goats/sheep, poultry, fish, shellfish, and game.	Meats
D002853	Chromatography, Liquid	Chromatographic techniques in which the mobile phase is a liquid.	Liquid Chromatography
D003386	Brachyura	An infraorder of chiefly marine, largely carnivorous CRUSTACEA, in the order DECAPODA, including the genera Cancer, Uca, and Callinectes.	Blue Crab,Callinectes sapidus,Carcinus maenas,Crab, Blue,Crab, Common Shore,Crab, Green,Crabs, Short-Tailed,Crabs, True,Green Crab,Uca,Common Shore Crab,European Shore Crab,Blue Crabs,Brachyuras,Carcinus maena,Common Shore Crabs,Crab, European Shore,Crab, Short-Tailed,Crab, True,Crabs, Blue,Crabs, Common Shore,Crabs, Green,Crabs, Short Tailed,Green Crabs,Shore Crab, Common,Shore Crab, European,Shore Crabs, Common,Short-Tailed Crab,Short-Tailed Crabs,True Crab,True Crabs,Ucas,maenas, Carcinus
D006604	Hexylresorcinol	A substituted dihydroxybenzene used topically as an antiseptic for the treatment of minor skin infections.	4-Hexylresorcinol,4 Hexylresorcinol
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia