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When Corynebacterium glutamicum is grown with a sufficient nitrogen supply, urea crosses the cytoplasmic membrane by passive diffusion. A permeability coefficient for urea diffusion of 9 x 10(-7) cm s-1 was determined. Under conditions of nitrogen starvation, an energy-dependent urea uptake system was synthesized. Carrier-mediated urea transport was catalyzed by a secondary transport system linked with proton motive force. With a Km for urea of 9 microM, the affinity of this uptake system was much higher than the affinity of urease towards its substrate (Km approximately 55 mM urea). The maximum uptake velocity depended on the expression level and was relatively low [2-3.5 nmol min-1 (mg dry wt.)-1].
R M Siewe, and
B Weil, and
A Burkovski, and
L Eggeling, and
R Krämer, and
T Jahns
November 2004,
Journal of bacteriology,
R M Siewe, and
B Weil, and
A Burkovski, and
L Eggeling, and
R Krämer, and
T Jahns
August 2000,
FEMS microbiology letters,
R M Siewe, and
B Weil, and
A Burkovski, and
L Eggeling, and
R Krämer, and
T Jahns
January 2000,
DNA sequence : the journal of DNA sequencing and mapping,
R M Siewe, and
B Weil, and
A Burkovski, and
L Eggeling, and
R Krämer, and
T Jahns
December 1990,
Journal of bacteriology,
R M Siewe, and
B Weil, and
A Burkovski, and
L Eggeling, and
R Krämer, and
T Jahns
January 1991,
FEMS microbiology letters,
R M Siewe, and
B Weil, and
A Burkovski, and
L Eggeling, and
R Krämer, and
T Jahns
July 2020,
World journal of microbiology & biotechnology,
R M Siewe, and
B Weil, and
A Burkovski, and
L Eggeling, and
R Krämer, and
T Jahns
August 1995,
Journal of bacteriology,
R M Siewe, and
B Weil, and
A Burkovski, and
L Eggeling, and
R Krämer, and
T Jahns
March 2015,
Applied microbiology and biotechnology,
R M Siewe, and
B Weil, and
A Burkovski, and
L Eggeling, and
R Krämer, and
T Jahns
April 1956,
Journal of general microbiology,
R M Siewe, and
B Weil, and
A Burkovski, and
L Eggeling, and
R Krämer, and
T Jahns
March 2011,
Biotechnology journal,
