Differential in situ expression of alpha2(XI) collagen mRNA isoforms in the developing mouse. 1998

M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
Department of Orthopaedic Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita 565, Japan. sugimoto@patho.med.osaka-u.ac.jp

Type XI collagen is an essential structural component of the extracellular matrix of cartilage and plays a role in collagen fibril formation and skeletal morphogenesis. The expression of all three type XI collagen genes is not restricted to cartilage. In addition, alternative exon usage seems to increase the structural diversity and functional potential of type XI collagen during development. In order to investigate type XI collagen expression during development, we have examined alpha2(XI) and alpha1(XI) collagen genes by in situ hybridization in mice. Transcripts of the alpha2(XI) collagen gene were first detected in the notochord of mouse embryos after 11.5 days of gestation. Subsequently, alpha2(XI) mRNA was mainly found in the cartilaginous tissues of the developing limbs and axial skeleton together with transcripts of the alpha1(XI) gene. The alpha2(XI) transcripts seemed to be alternatively spliced isoforms lacking exons 6-8, which code for an acidic domain. Expression of alpha2(XI) outside the cartilage was relatively restricted, whereas expression of the alpha1(XI) gene was widespread. However, expression of alpha2(XI) transcripts containing exons 6-8 was found in non-chondrogenic tissues, including the calvarium and periosteum where intramembranous ossification occurs. These results indicate that alpha2(XI) mRNA isoforms are differentially expressed in various tissues during development. In addition, alpha2(XI) mRNA isoforms containing alternative exons are present in osteogenic cells, and their expression may be closely related to the formation of bone or cartilage.

UI MeSH Term Description Entries
D007536 Isomerism The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed) Isomerisms
D008813 Mice, Inbred ICR An inbred strain of mouse that is used as a general purpose research strain, for therapeutic drug testing, and for the genetic analysis of CARCINOGEN-induced COLON CANCER. Mice, Inbred ICRC,Mice, ICR,Mouse, ICR,Mouse, Inbred ICR,Mouse, Inbred ICRC,ICR Mice,ICR Mice, Inbred,ICR Mouse,ICR Mouse, Inbred,ICRC Mice, Inbred,ICRC Mouse, Inbred,Inbred ICR Mice,Inbred ICR Mouse,Inbred ICRC Mice,Inbred ICRC Mouse
D001842 Bone and Bones A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principal cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX. Bone Tissue,Bone and Bone,Bone,Bones,Bones and Bone,Bones and Bone Tissue,Bony Apophyses,Bony Apophysis,Condyle,Apophyses, Bony,Apophysis, Bony,Bone Tissues,Condyles,Tissue, Bone,Tissues, Bone
D002356 Cartilage A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE. Cartilages
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005314 Embryonic and Fetal Development Morphological and physiological development of EMBRYOS or FETUSES. Embryo and Fetal Development,Prenatal Programming,Programming, Prenatal
D005552 Forelimb A front limb of a quadruped. (The Random House College Dictionary, 1980) Forelimbs
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated

Related Publications

M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
January 2005, Journal of bone and mineral metabolism,
M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
February 1994, Journal of neuroscience research,
M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
October 2007, The Journal of comparative neurology,
M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
February 2001, Matrix biology : journal of the International Society for Matrix Biology,
M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
February 1999, Muscle & nerve,
M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
February 2010, Biochemical and biophysical research communications,
M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
April 2004, Acta oto-laryngologica,
M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
May 2007, International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience,
M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
March 1998, The Journal of pathology,
M Sugimoto, and T Kimura, and N Tsumaki, and Y Matsui, and K Nakata, and H Kawahata, and N Yasui, and Y Kitamura, and S Nomura, and T Ochi
September 1998, The Journal of biological chemistry,
Copied contents to your clipboard!