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A novel chromatographic process for purification of alpha 1 proteinase inhibitor (alpha 1-PI) from Cohn fraction IV-1 paste is described. This process has been successfully scaled up to 50-1 columns. It involves DEAE chromatography, sulfopropyl (S) cation chromatography, tri-n-butyl phosphate (TNBP)-cholate treatment, a second S cation chromatography, freeze-drying and dry-heat. The process has been optimized for purity, yield, lipid removal, chemical usage and water consumption. Filtration after TNBP-cholate treatment plays a key role in ensuring a low lipid content in the final product. Pre-equilibration with high salt buffer is necessary to reduce the water consumption significantly during the ion-exchange chromatography equilibration step. The final product is approximately 95% pure by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with a 64% to 70% yield from IV-1 paste.
S X Chen, and
D J Hammond, and
A M Klos, and
W D Wood, and
J E Wydick, and
W R Lebing
May 1978,
Biochimica et biophysica acta,
S X Chen, and
D J Hammond, and
A M Klos, and
W D Wood, and
J E Wydick, and
W R Lebing
December 1980,
Biochimica et biophysica acta,
S X Chen, and
D J Hammond, and
A M Klos, and
W D Wood, and
J E Wydick, and
W R Lebing
January 1975,
Preparative biochemistry,
S X Chen, and
D J Hammond, and
A M Klos, and
W D Wood, and
J E Wydick, and
W R Lebing
October 1962,
Archives of biochemistry and biophysics,
S X Chen, and
D J Hammond, and
A M Klos, and
W D Wood, and
J E Wydick, and
W R Lebing
January 2007,
Advances in experimental medicine and biology,
S X Chen, and
D J Hammond, and
A M Klos, and
W D Wood, and
J E Wydick, and
W R Lebing
March 2010,
P & T : a peer-reviewed journal for formulary management,
S X Chen, and
D J Hammond, and
A M Klos, and
W D Wood, and
J E Wydick, and
W R Lebing
November 1990,
Journal of chromatography,
S X Chen, and
D J Hammond, and
A M Klos, and
W D Wood, and
J E Wydick, and
W R Lebing
January 2019,
PloS one,
S X Chen, and
D J Hammond, and
A M Klos, and
W D Wood, and
J E Wydick, and
W R Lebing
December 1996,
Chest,
S X Chen, and
D J Hammond, and
A M Klos, and
W D Wood, and
J E Wydick, and
W R Lebing
January 2007,
Advances in experimental medicine and biology,
