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Control of mutation frequency by bacteriophage T4 DNA polymerase. II. Accuracy of nucleotide selection by the L88 mutator, CB120 antimutator, and wild type phage T4 DNA polymerases. 1976

F D Gillin, and N G Nossal

The accuracy of nucleotide selection by wild type, L88 mutator, and CB120 antimutator T4 DNA polymerases has been compared by measuring both stable incorporation of complementary and noncomplementary nucleotides into polymer and the DNA-dependent conversion of deoxynucleoside triphosphate to monophosphate. The increased accuracy of the CB120 antimutator enzyme is shown by a ratio of utilization of incorrect to correct nucleotides with poly(dA)-poly(dT) as template which is only 10 to 30% of that of the wild type enzyme. In contrast, the ratio of incorrect to correct nucleotide utilized by the L88 mutator enzyme with this template was higher than that of the wild type enzyme, in agreement with the report of Hershfield (Hershfield, M.S. (1973) J. Biol, Chem. 248, 1417-1423). The antimutator, mutator, and wild type enzymes each have a much higher apparent Km for the noncomplementary nucleotides than for complementary nucleotides with this template. The L88 mutator polymerase has a higher "Km" for poly(dA)-poly(dT) than the wild type enzyme in reactions with both complementary and noncomplementary nucleotides. The antimutator polymerase has an elevated "Km" for polymer only for reactions with noncomplementary nucleotides. The wild type and L88 mutator enzymes also utilized both noncomplementary nucleotides much more frequently than the CB120 antimutator enzymes with poly [d(A-T)] as the template-primer. The T4 gene 32DNA unwinding protein appears to facilitate the correct reading of this template since it decreases the ratio of incorrect nucleotides utilized by both the wild type and L88 mutator polymerases.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008345 Manganese A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D003090 Coliphages Viruses whose host is Escherichia coli. Escherichia coli Phages,Coliphage,Escherichia coli Phage,Phage, Escherichia coli,Phages, Escherichia coli
D004254 DNA Nucleotidyltransferases Enzymes that catalyze the incorporation of deoxyribonucleotides into a chain of DNA. EC 2.7.7.-. Nucleotidyltransferases, DNA
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species

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