-transparent.png){kind=logo}
OBJECTIVE To assess the relationship between in vivo measurements of optic disc topography and histomorphometric measurements of optic nerve fiber number in glaucoma. METHODS Both eyes of 10 monkeys (Macaca fascicularis) with laser-induced glaucoma in the right eye were studied. Optic disc topography was measured in vivo with a confocal scanning laser ophthalmoscope. Histomorphometry was performed on optic nerve cross sections using bright-field microscopy with camera lucida. Nerve fiber density was estimated by unbiased random sampling. Nerve fiber number was estimated for each sector by multiplying nerve fiber density with neuroglial area. Nerve fiber count was compared with each of 13 global optic disc topographic parameters. RESULTS For neuroretinal measurements in the glaucomatous eyes, rim area, retinal nerve fiber layer (RNFL) cross-sectional area, rim volume, and RNFL thickness correlated significantly with optic nerve fiber number. Differences in nerve fiber count between control and glaucomatous optic nerves showed the strongest correlation with differences in mean height contour; this was followed by RNFL cross-sectional area, RNFL thickness, rim volume, and differences in rim area. For cup measurements in the glaucomatous eyes, cup volume below reference, cup area, mean cup depth, the ratio of cup area to disc area, and cup shape correlated significantly with nerve fiber number. Differences in nerve fiber number between control and glaucomatous optic nerves showed the strongest correlation with differences in cup shape; this was followed by mean cup depth, cup volume below reference, the ratio of cup area to disc area, cup area, and differences in cup volume below surface. No association was found between optic nerve fiber number and optic disc area in glaucomatous eyes. CONCLUSIONS In experimental glaucoma, most optic disc topography measures correlated significantly with optic nerve fiber number. The results of this histomorphometric study support the use of confocal scanning laser ophthalmoscopy to evaluate optic nerve damage in glaucoma.
Y H Yücel, and
N Gupta, and
M W Kalichman, and
A P Mizisin, and
W Hare, and
M de Souza Lima, and
L Zangwill, and
R N Weinreb
February 1977,
Journal of the American Optometric Association,
Y H Yücel, and
N Gupta, and
M W Kalichman, and
A P Mizisin, and
W Hare, and
M de Souza Lima, and
L Zangwill, and
R N Weinreb
August 2006,
Ophthalmology,
Y H Yücel, and
N Gupta, and
M W Kalichman, and
A P Mizisin, and
W Hare, and
M de Souza Lima, and
L Zangwill, and
R N Weinreb
March 2020,
Journal of pediatric ophthalmology and strabismus,
Y H Yücel, and
N Gupta, and
M W Kalichman, and
A P Mizisin, and
W Hare, and
M de Souza Lima, and
L Zangwill, and
R N Weinreb
February 1986,
Nippon Ganka Gakkai zasshi,
Y H Yücel, and
N Gupta, and
M W Kalichman, and
A P Mizisin, and
W Hare, and
M de Souza Lima, and
L Zangwill, and
R N Weinreb
June 2005,
Acta ophthalmologica Scandinavica,
Y H Yücel, and
N Gupta, and
M W Kalichman, and
A P Mizisin, and
W Hare, and
M de Souza Lima, and
L Zangwill, and
R N Weinreb
January 1996,
International ophthalmology,
Y H Yücel, and
N Gupta, and
M W Kalichman, and
A P Mizisin, and
W Hare, and
M de Souza Lima, and
L Zangwill, and
R N Weinreb
August 2010,
[Zhonghua yan ke za zhi] Chinese journal of ophthalmology,
Y H Yücel, and
N Gupta, and
M W Kalichman, and
A P Mizisin, and
W Hare, and
M de Souza Lima, and
L Zangwill, and
R N Weinreb
August 1997,
Journal of glaucoma,
Y H Yücel, and
N Gupta, and
M W Kalichman, and
A P Mizisin, and
W Hare, and
M de Souza Lima, and
L Zangwill, and
R N Weinreb
August 1988,
Investigative ophthalmology & visual science,
Y H Yücel, and
N Gupta, and
M W Kalichman, and
A P Mizisin, and
W Hare, and
M de Souza Lima, and
L Zangwill, and
R N Weinreb
November 2005,
Optometry (St. Louis, Mo.),
