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We developed a new simple sandwich-type enzyme immunoassay to measure cholesteryl ester transfer protein (CETP) mass in human serum. In assay validation, Intra- and Inter-assay coefficients of variation were 2.7 to 5.7% and 2.2 to 12.2%, respectively. There was no cross-reactivity with various lipoproteins (apo A-I, apo A-II, apo B, apo C-III). A good correlation between CETP mass and CETP activity (n = 46, correlation coefficient = 0.88) was observed. This assay provided a specific and reproducible method for measuring CETP mass in samples. The average value of CETP in the normal sera of 41 males was 1.8+/-0.6 microg/ml (mean+/-S.D.) and that of 37 females was 2.0+/-0.5 microg/ml. In the study of patients with the CETP gene mutation (Int 14A and D442G), our results on the value of plasma CETP mass reflected to genetic CETP deficiency. In conclusion, this assay for CETP mass in human serum may be a useful tool for clinical investigations involving lipid metabolism related to disease.
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T Kiyohara, and
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International journal of clinical & laboratory research,
T Kiyohara, and
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July 1998,
Clinical chemistry,
T Kiyohara, and
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A Inazu, and
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K Chichibu
July 1978,
Proceedings of the National Academy of Sciences of the United States of America,
T Kiyohara, and
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K Chichibu
September 1991,
Lancet (London, England),
T Kiyohara, and
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K Chichibu
June 1999,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
T Kiyohara, and
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H Mabuchi, and
K Chichibu
June 2002,
Obesity research,
T Kiyohara, and
R Kiriyama, and
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A Inazu, and
J Koizumi, and
H Mabuchi, and
K Chichibu
May 2023,
Journal of chemical information and modeling,
