Biomaterial Database

[Kinetic determination of urea with the LKB system (author's transl)]. 1976

H G Eisenwiener

The kinetic determination of urea based on the urea/glutamate dehydrogenase method was adapted for the LKB Reaction Rate Analyzer System 8600. A ratio of sample to reagent volume of 1:50 ensures linearity up to 33.3 mmol/l with a day to day precision of 5%. Parallel studies with the urease/glutamate dehydrogenase method were performed with the CentrifiChem System and with the manual Berthelot/Salicylate method.

UI	MeSH Term	Description	Entries
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D005969	Glutamate Dehydrogenase	An enzyme that catalyzes the conversion of L-glutamate and water to 2-oxoglutarate and NH3 in the presence of NAD+. (From Enzyme Nomenclature, 1992) EC 1.4.1.2.	Dehydrogenase, Glutamate
D001322	Autoanalysis	Method of analyzing chemicals using automation.	Autoanalyses
D014508	Urea	A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.	Basodexan,Carbamide,Carmol
D014510	Urease	An enzyme that catalyzes the conversion of urea and water to carbon dioxide and ammonia. EC 3.5.1.5.	Phytourease,Urea Amidohydrolase,Amidohydrolase, Urea