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BACKGROUND The cyclin D1 gene is amplified and/or overexpressed in several types of human cancer, including cancers of the breast, esophagus, head, and neck. However, the role of cyclin D1 in prostate cancer has not been previously studied in detail. METHODS Six human prostate cancer cell lines and cultures of normal human prostate cells were examined by Western and Northern blot analyses for levels of expression of the cyclin D1 protein and mRNA, respectively. Southern blot analyses were performed to examine possible amplification of this gene. Immunostaining for cyclin D1 was performed on 50 primary prostate cancer samples. RESULTS Cyclin D1 protein was expressed at relatively high levels in all of the six human prostate cancer cell lines examined, but was not detected in the cultures of normal human prostate cells. The ALVA 41 cell line expressed the highest level of this protein. Relatively high levels of cyclin D1 mRNA were also found in all of the prostate cancer cell lines. Nevertheless, none of these cell lines revealed amplification of the cyclin D1 gene. Twelve of the 50 primary prostate cancer samples (24%) revealed regions of moderate to strongly positive staining for cyclin D1. CONCLUSIONS The increased expression of cyclin D1 in several prostate cancer cell lines and in a subset of primary prostate cancer samples suggests that further studies on the expression of this gene and related genes may be of interest in understanding the pathogenesis of prostate cancer.
E K Han, and
J T Lim, and
N Arber, and
M A Rubin, and
W Q Xing, and
I B Weinstein
June 2014,
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
E K Han, and
J T Lim, and
N Arber, and
M A Rubin, and
W Q Xing, and
I B Weinstein
June 1999,
International journal of cancer,
E K Han, and
J T Lim, and
N Arber, and
M A Rubin, and
W Q Xing, and
I B Weinstein
January 2005,
Prostate cancer and prostatic diseases,
E K Han, and
J T Lim, and
N Arber, and
M A Rubin, and
W Q Xing, and
I B Weinstein
January 1995,
Urologic oncology,
E K Han, and
J T Lim, and
N Arber, and
M A Rubin, and
W Q Xing, and
I B Weinstein
January 1999,
The Prostate,
E K Han, and
J T Lim, and
N Arber, and
M A Rubin, and
W Q Xing, and
I B Weinstein
June 2001,
Cancer,
E K Han, and
J T Lim, and
N Arber, and
M A Rubin, and
W Q Xing, and
I B Weinstein
October 2003,
International journal of oral and maxillofacial surgery,
E K Han, and
J T Lim, and
N Arber, and
M A Rubin, and
W Q Xing, and
I B Weinstein
January 2002,
Oncology reports,
E K Han, and
J T Lim, and
N Arber, and
M A Rubin, and
W Q Xing, and
I B Weinstein
January 1996,
Journal of experimental therapeutics & oncology,
E K Han, and
J T Lim, and
N Arber, and
M A Rubin, and
W Q Xing, and
I B Weinstein
August 1995,
Cancer,
