BIOMAT Database Logo BIOMAT Database Logo

Detection of Yersinia enterocolitica in food by PCR amplification. 1998

A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
National Food Administration, Swedish University of Agricultural Sciences, Uppsala, Sweden.

A polymerase chain reaction (PCR) assay was developed for detection of pathogenic, virulent strains of Yersinia enterocolitica. By using both virulence loci virF and ail as markers for pathogenicity, detection of species with a virulence factor present was possible. DNA preparation in the presence of hexadecyl trimethy ammonium bromide (CTAB) was followed by two 44 cycle amplification reactions, one for each of the markers. As few as 10(2) Y. enterocolitica cells were detected in ground pork in the presence of 10(5)-10(6) bacteria of other species. The described PCR assay provides a sensitive robust assay for the detection of virulent Y. enterocolitica in food.

UI MeSH Term Description Entries
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D015008 Yersinia enterocolitica A species of the genus YERSINIA, isolated from both man and animal. It is a frequent cause of bacterial gastroenteritis in children. Bacterium enterocoliticum
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain

Related Publications

A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
Detection of pathogenic Yersinia enterocolitica in environmental waters by PCR.
March 1996, The Journal of applied bacteriology,
A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
[Detection of Yersinia enterocolitica in diarrhea stool by real-time PCR].
August 2006, Zhonghua yi xue za zhi,
A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
Detection of Yersinia enterocolitica in food: an overview.
April 2015, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes.
September 2010, Journal of water and health,
A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
Detection of pathogenic Yersinia enterocolitica by a swab enrichment PCR procedure.
January 2003, Methods in molecular biology (Clifton, N.J.),
A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
Detection of Yersinia enterocolitica serogroup O:3 by a PCR method.
May 1996, Journal of clinical microbiology,
A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
Food-PCR. Validation and standardization of diagnostic PCR for detection of Yersinia enterocolitica and other foodborne pathogens.
January 2003, Advances in experimental medicine and biology,
A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
Yersinia enterocolitica in food hygiene.
January 1991, International journal of food microbiology,
A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay.
December 2001, Journal of clinical microbiology,
A Nilsson, and S T Lambertz, and P Stålhandske, and P Norberg, and M L Danielsson-Tham
Detection and enumeration of virulent Yersinia enterocolitica in food by DNA colony hybridization.
September 1983, Applied and environmental microbiology,
Copied contents to your clipboard!