Synthesis and regulation of leukaemia inhibitory factor in cultured bovine oviduct cells by hormones. 1998

K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
Department of Obstetrics and Gynecology, Clinic for Endocrinology, University Hospital, Zurich, Switzerland.

Leukaemia inhibitory factor (LIF) is an essential factor for embryo implantation. Factors generated by the oviduct cells (epithelial cells and fibroblasts) create the microenvironment for fertilization and first embryo stage development. Hence, it is feasible that the oviduct cells also synthesize LIF to promote and condition the embryo for implantation in the uterus. In the present study, we investigated whether cultured bovine oviduct epithelial cells and fibroblasts synthesize LIF. LIF production was measured in the conditioned medium of oviduct epithelial cells and fibroblasts, using LIF enzyme-linked immunosorbent assay. Moreover, expression of LIF mRNA was confirmed by LIF reverse transcriptase-polymerase chain reaction in extracts of RNA from oviduct epithelial/fibroblast cells. Quantitatively similar amounts of LIF were detected in the culture medium of epithelial cells and fibroblasts. In cells cultured for 1-7 days, the levels of LIF in the medium increased in a time-dependent manner. As compared to untreated cells, treatment of cells with 17beta-oestradiol (1-100 ng/ ml), but not progesterone (1-100 ng/ml) and insulin (20 ng/ml), increased the levels of LIF in a concentration-dependent manner (P < 0.05). Similarly, tumour necrosis factor-alpha (100 ng/ml) significantly induced the levels of LIF. The effects of 17beta-oestradiol (50 ng/ml) on LIF synthesis were enhanced and not blocked in the presence of tamoxifen (1 microg/ml), an oestrogen receptor antagonist, suggesting that the stimulatory effects of 17beta-oestradiol on LIF synthesis are not receptor-mediated. In conclusion 17beta-oestradiol, but not progesterone, induces LIF synthesis by bovine oviduct epithelial cells and fibroblasts and this may play an important role in the biology of early embryo development. However, the exact pathophysiological role of LIF within the oviduct needs to be further investigated.

UI MeSH Term Description Entries
D007328 Insulin A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1). Iletin,Insulin A Chain,Insulin B Chain,Insulin, Regular,Novolin,Sodium Insulin,Soluble Insulin,Chain, Insulin B,Insulin, Sodium,Insulin, Soluble,Regular Insulin
D008222 Lymphokines Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity. Lymphocyte Mediators,Mediators, Lymphocyte
D011374 Progesterone The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS. Pregnenedione,Progesterone, (13 alpha,17 alpha)-(+-)-Isomer,Progesterone, (17 alpha)-Isomer,Progesterone, (9 beta,10 alpha)-Isomer
D011500 Protein Synthesis Inhibitors Compounds which inhibit the synthesis of proteins. They are usually ANTI-BACTERIAL AGENTS or toxins. Mechanism of the action of inhibition includes the interruption of peptide-chain elongation, the blocking the A site of ribosomes, the misreading of the genetic code or the prevention of the attachment of oligosaccharide side chains to glycoproteins. Protein Synthesis Antagonist,Protein Synthesis Antagonists,Protein Synthesis Inhibitor,Antagonist, Protein Synthesis,Antagonists, Protein Synthesis,Inhibitor, Protein Synthesis,Inhibitors, Protein Synthesis,Synthesis Antagonist, Protein,Synthesis Inhibitor, Protein
D011960 Receptors, Estrogen Cytoplasmic proteins that bind estrogens and migrate to the nucleus where they regulate DNA transcription. Evaluation of the state of estrogen receptors in breast cancer patients has become clinically important. Estrogen Receptor,Estrogen Receptors,Estrogen Nuclear Receptor,Estrogen Receptor Type I,Estrogen Receptor Type II,Estrogen Receptors Type I,Estrogen Receptors Type II,Receptor, Estrogen Nuclear,Receptors, Estrogen, Type I,Receptors, Estrogen, Type II,Nuclear Receptor, Estrogen,Receptor, Estrogen
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002614 Chelating Agents Chemicals that bind to and remove ions from solutions. Many chelating agents function through the formation of COORDINATION COMPLEXES with METALS. Chelating Agent,Chelator,Complexons,Metal Antagonists,Chelators,Metal Chelating Agents,Agent, Chelating,Agents, Chelating,Agents, Metal Chelating,Antagonists, Metal,Chelating Agents, Metal
D003513 Cycloheximide Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis. Actidione,Cicloheximide

Related Publications

K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
September 1995, In vitro cellular & developmental biology. Animal,
K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
January 1992, Ciba Foundation symposium,
K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
May 1994, Journal of immunology (Baltimore, Md. : 1950),
K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
December 2006, Journal of physiology and pharmacology : an official journal of the Polish Physiological Society,
K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
September 1994, Biology of reproduction,
K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
March 1987, Endocrinology,
K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
January 2000, The Journal of biological chemistry,
K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
May 1989, Journal of animal science,
K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
August 1995, Journal of neuroendocrinology,
K C Reinhart, and R K Dubey, and C L Mummery, and M van Rooijen, and P J Keller, and R Marinella
August 2011, The Biochemical journal,
Copied contents to your clipboard!