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1. Precision-cut liver slices represent a suitable and convenient in vitro preparation for studying metabolism and toxicity mechanisms of drugs and toxic chemicals. Particularly in the case of human liver slices, cryopreservation would enable more efficient utilization of this scarce and irregularly available tissue. 2. Liver slices from consecutive human livers were cryopreserved using a method previously developed for rat and monkey liver slices. This procedure involves incubation in 12% dimethyl sulphoxide for 30 min on ice and direct immersion into liquid nitrogen. 3. Functional integrity of cryopreserved human liver slices, as compared with that of fresh liver slices, was maintained at 66 +/- 8% (alanine aminotransferase activity retained in the slices), 78 +/- 7% (urea synthesis), 88 +/- 14% (testosterone hydroxylation), 84 +/- 7% (N-deethylation of lidocaine) and 88 +/- 10% (total O-deethylation of 7-ethoxycoumarin). The ratios of testosterone metabolites did not change on cryopreservation. 4. These results show that the cryopreserved human liver slices retained the measured drug metabolism activities. Therefore, this cryopreservation method is suitable for storing liver slices to be used for comparing drug metabolism patterns, at least qualitatively, between species.
R de Kanter, and
P Olinga, and
I Hof, and
M de Jager, and
W A Verwillegen, and
M J Slooff, and
H J Koster, and
D K Meijer, and
G M Groothuis
March 1999,
Cryobiology,
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P Olinga, and
I Hof, and
M de Jager, and
W A Verwillegen, and
M J Slooff, and
H J Koster, and
D K Meijer, and
G M Groothuis
September 2007,
Parasitology research,
R de Kanter, and
P Olinga, and
I Hof, and
M de Jager, and
W A Verwillegen, and
M J Slooff, and
H J Koster, and
D K Meijer, and
G M Groothuis
April 1991,
Cryobiology,
R de Kanter, and
P Olinga, and
I Hof, and
M de Jager, and
W A Verwillegen, and
M J Slooff, and
H J Koster, and
D K Meijer, and
G M Groothuis
June 1986,
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde,
R de Kanter, and
P Olinga, and
I Hof, and
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H J Koster, and
D K Meijer, and
G M Groothuis
June 1993,
Cryobiology,
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P Olinga, and
I Hof, and
M de Jager, and
W A Verwillegen, and
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H J Koster, and
D K Meijer, and
G M Groothuis
October 2009,
The International journal of artificial organs,
R de Kanter, and
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September 1981,
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January 1977,
Transfusion,
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November 2021,
Histochemistry and cell biology,
R de Kanter, and
P Olinga, and
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W A Verwillegen, and
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January 2002,
Toxicology mechanisms and methods,
