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A region-specific microdissection library originating from human chromosome 17q21, was constructed using the MboI linker-adaptor microcloning technique. DNA sequencing of 241 microclones resulted in the identification of 74 novel coding sequences, paralogs of known genes, and known, but previously unmapped, genes or expressed sequence tags that were "virtually" mapped to chromosome 17q21. By pooling the microclones as multiplexed hybridization probes, and by virtue of their origin on 17q21, we were able to identify approximately 150 P1 clones from the human Reference Library Data Base P1 Library that potentially map to chromosome 17q21. Verification of the 17q21 location of 16 P1 clones was accomplished by PCR analysis with STS primer pairs to known 17q21 genes or by FISH. Our results demonstrate the substantial advantage of combining the sequence analysis of microclones with multiplex hybridization strategies for gene discovery and mapping specific gene rich regions of the genome.
K L Bentley, and
W L Li, and
F O VannBerg, and
J Y Choi, and
J Yu, and
F T Kao, and
G Ruaño
March 1992,
Nucleic acids research,
K L Bentley, and
W L Li, and
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F T Kao, and
G Ruaño
August 1993,
Genomics,
K L Bentley, and
W L Li, and
F O VannBerg, and
J Y Choi, and
J Yu, and
F T Kao, and
G Ruaño
January 1993,
Cytogenetics and cell genetics,
K L Bentley, and
W L Li, and
F O VannBerg, and
J Y Choi, and
J Yu, and
F T Kao, and
G Ruaño
November 1992,
Genomics,
K L Bentley, and
W L Li, and
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J Y Choi, and
J Yu, and
F T Kao, and
G Ruaño
November 1992,
Genomics,
K L Bentley, and
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F O VannBerg, and
J Y Choi, and
J Yu, and
F T Kao, and
G Ruaño
January 1995,
Cytogenetics and cell genetics,
K L Bentley, and
W L Li, and
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G Ruaño
March 1995,
Mammalian genome : official journal of the International Mammalian Genome Society,
K L Bentley, and
W L Li, and
F O VannBerg, and
J Y Choi, and
J Yu, and
F T Kao, and
G Ruaño
March 1994,
Somatic cell and molecular genetics,
K L Bentley, and
W L Li, and
F O VannBerg, and
J Y Choi, and
J Yu, and
F T Kao, and
G Ruaño
May 1994,
Human genetics,
K L Bentley, and
W L Li, and
F O VannBerg, and
J Y Choi, and
J Yu, and
F T Kao, and
G Ruaño
January 1994,
Genomics,
