Biomaterial Database

Development of an optimal method for the dual perfusion of the isolated rat liver. 1998

S Sahin, and M Rowland

School of Pharmacy and Pharmaceutical Sciences, University of Manchester, UK.

The liver receives two blood supplies, the portal vein (PV) and hepatic artery (HA). The isolated perfused rat liver preparation (IPRL) is widely used to examine physiological factors affecting the hepatic disposition of compounds, but usually it is perfused via the PV only. In the development of a more physiological dual perfused system, we examined three surgical procedures for HA perfusion--cannulation through the gastroduodenal artery, the aorta, and the celiac artery--using 14C-urea as the reference marker. Similar efflux profiles for 14C-urea were obtained for all three procedures, with a clear difference between HA and PV administration; however, cannulation of blood vessels and isolation of the HA supply were the most reliable with the celiac artery cannulation, making it the preferred procedure.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D010477	Perfusion	Treatment process involving the injection of fluid into an organ or tissue.	Perfusions
D011169	Portal Vein	A short thick vein formed by union of the superior mesenteric vein and the splenic vein.	Portal Veins,Vein, Portal,Veins, Portal
D002250	Carbon Radioisotopes	Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.	Radioisotopes, Carbon
D002445	Celiac Artery	The arterial trunk that arises from the abdominal aorta and after a short course divides into the left gastric, common hepatic and splenic arteries.	Arteries, Celiac,Artery, Celiac,Celiac Arteries
D006499	Hepatic Artery	A branch of the celiac artery that distributes to the stomach, pancreas, duodenum, liver, gallbladder, and greater omentum.	Arteries, Hepatic,Artery, Hepatic,Hepatic Arteries
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001011	Aorta	The main trunk of the systemic arteries.	Aortas
D014508	Urea	A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.	Basodexan,Carbamide,Carmol