-transparent.png){kind=logo}
Entamoeba histolytica HMI:IMSS trophozoites were able to utilize human hemoglobin but not hemin as a sole iron source to grow in vitro. Proteases from crude extracts of E. histolytica degraded human, porcine, and bovine hemoglobins at pH 7.0. These proteolytic activities were found by electrophoresis in SDS-polyacrylamide gels copolymerized with hemoglobin, with apparent molecular weights of 116, 82, and 21 kDa, the 82-kDa protein being the most active protease against this substrate. The proteases were classified in the cysteine group since the activities were inhibited by l-trans-epoxysuccinylleucylamido(4-guanidino)butane, p-hydroxymercuribenzoate, iodoacetate, and N-ethylmaleimide and activated with dithiothreitol. Other pathogenic strains of E. histolytica showed the same pattern of hemoglobinases. These hemoglobin-degrading proteases could be playing an important role in iron acquisition by E. histolytica.
J J Serrano-Luna, and
E Negrete, and
M Reyes, and
M de la Garza
January 2013,
PloS one,
J J Serrano-Luna, and
E Negrete, and
M Reyes, and
M de la Garza
January 1986,
Archivos de investigacion medica,
J J Serrano-Luna, and
E Negrete, and
M Reyes, and
M de la Garza
December 2002,
The American journal of tropical medicine and hygiene,
J J Serrano-Luna, and
E Negrete, and
M Reyes, and
M de la Garza
April 2010,
Planta medica,
J J Serrano-Luna, and
E Negrete, and
M Reyes, and
M de la Garza
January 1992,
Archives of medical research,
J J Serrano-Luna, and
E Negrete, and
M Reyes, and
M de la Garza
December 1985,
International journal for parasitology,
J J Serrano-Luna, and
E Negrete, and
M Reyes, and
M de la Garza
July 2010,
Experimental parasitology,
J J Serrano-Luna, and
E Negrete, and
M Reyes, and
M de la Garza
December 1997,
Molecular and biochemical parasitology,
J J Serrano-Luna, and
E Negrete, and
M Reyes, and
M de la Garza
January 2018,
The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale,
J J Serrano-Luna, and
E Negrete, and
M Reyes, and
M de la Garza
March 2006,
Gastroenterology,
