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Further biochemical investigations were performed in a case of glucose-6-phosphate dehydrogenase deficiency--called the "Vienna" deficiency variant on account of its biochemical characterization. It was found that the "Vienna" deficiency variant shows increased enzyme protein at a reduced specific activity. Gel filtration on Sephadex-G-200 produced a molecular weight identical with that of the normoenzyme; the different cochromatographic behaviour on hydroxylapatite appears attributable to a sequence modification of the amino acids.
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