Biomaterial Database

Molecular cloning and sequencing of the rat interleukin-12 p40 gene. 1998

J Khalife, and C Pierrot, and C Cocude, and C Cêtre, and C Godin, and A Capron

Centre d'Immunologie et de Biologie Parasitaire, INSERM U. 167, Institut Pasteur, Lille, France.

The nucleotide sequences containing the rat interleukin-12 p40 gene was determined. Sequencing revealed the presence of six exons and five introns. Analysis of the 5' non-coding region showed the presence of several possible sites involved in cytokine gene regulation at the transcriptional level. Comparison of the deduced amino acid sequence of rat IL-12 p40 with that of the mouse and of human p40, showed 92% and 65% identity respectively.

UI	MeSH Term	Description	Entries
D007438	Introns	Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.	Intervening Sequences,Sequences, Intervening,Intervening Sequence,Intron,Sequence, Intervening
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D005091	Exons	The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.	Mini-Exon,Exon,Mini Exon,Mini-Exons
D005786	Gene Expression Regulation	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.	Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005796	Genes	A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.	Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D001665	Binding Sites	The parts of a macromolecule that directly participate in its specific combination with another molecule.	Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining

Related Publications

J Khalife, and C Pierrot, and C Cocude, and C Cêtre, and C Godin, and A Capron

Avian interleukin-12beta (p40): cloning and characterization of the cDNA and gene.

December 2003, Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research,

J Khalife, and C Pierrot, and C Cocude, and C Cêtre, and C Godin, and A Capron

Cloning and sequencing of horse interleukin-12 and interleukin-18 cDNAs.

October 1999, Immunogenetics,

J Khalife, and C Pierrot, and C Cocude, and C Cêtre, and C Godin, and A Capron

Molecular cloning of cat interleukin-12.

January 1997, Immunogenetics,

J Khalife, and C Pierrot, and C Cocude, and C Cêtre, and C Godin, and A Capron

The expression of interleukin-23 (p19/p40) and interleukin-12 (p35/p40) in psoriasis skin.

January 2006, Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban,

J Khalife, and C Pierrot, and C Cocude, and C Cêtre, and C Godin, and A Capron

Detection, cDNA cloning and sequencing of canine interleukin 12.

April 1998, Cytokine,

J Khalife, and C Pierrot, and C Cocude, and C Cêtre, and C Godin, and A Capron

The interleukin-12 subunit p40 specifically inhibits effects of the interleukin-12 heterodimer.

September 1993, European journal of immunology,

J Khalife, and C Pierrot, and C Cocude, and C Cêtre, and C Godin, and A Capron

Astrocytes and microglia produce interleukin-12 p40.

October 1996, Annals of the New York Academy of Sciences,

J Khalife, and C Pierrot, and C Cocude, and C Cêtre, and C Godin, and A Capron

The structure of interleukin-23 reveals the molecular basis of p40 subunit sharing with interleukin-12.

October 2008, Journal of molecular biology,