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Streptomyces griseus protease B, a member of the chymotrypsin superfamily, is encoded by a gene that express a pre-pro-mature protein. During secretion the precursor protein is processed into a mature, fully folded protease. In this study, we constructed a family of genes which encode deletions at the amino-terminal end of the propeptide. The secretion of active protease B was seen to decrease in an exponential manner according to the length of the deletion. The results underscore the intimate relationship between folding and secretion in bacterial protease expression. They further suggest that the propeptide segment of the zymogen stabilizes the folding of the mature through many small binding interactions over the entire surface of the peptide rather than through a few specific contacts.
J Baardsnes, and
S Sidhu, and
A MacLeod, and
J Elliott, and
D Morden, and
J Watson, and
T Borgford
February 1979,
Canadian journal of biochemistry,
J Baardsnes, and
S Sidhu, and
A MacLeod, and
J Elliott, and
D Morden, and
J Watson, and
T Borgford
February 1979,
International journal of peptide and protein research,
J Baardsnes, and
S Sidhu, and
A MacLeod, and
J Elliott, and
D Morden, and
J Watson, and
T Borgford
March 2000,
Chemistry & biology,
J Baardsnes, and
S Sidhu, and
A MacLeod, and
J Elliott, and
D Morden, and
J Watson, and
T Borgford
February 1994,
The Journal of biological chemistry,
J Baardsnes, and
S Sidhu, and
A MacLeod, and
J Elliott, and
D Morden, and
J Watson, and
T Borgford
September 1991,
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J Baardsnes, and
S Sidhu, and
A MacLeod, and
J Elliott, and
D Morden, and
J Watson, and
T Borgford
January 1978,
Biochemistry,
J Baardsnes, and
S Sidhu, and
A MacLeod, and
J Elliott, and
D Morden, and
J Watson, and
T Borgford
January 1967,
Ukrains'kyi biokhimichnyi zhurnal,
J Baardsnes, and
S Sidhu, and
A MacLeod, and
J Elliott, and
D Morden, and
J Watson, and
T Borgford
January 1973,
Ukrains'kyi biokhimichnyi zhurnal,
J Baardsnes, and
S Sidhu, and
A MacLeod, and
J Elliott, and
D Morden, and
J Watson, and
T Borgford
January 1970,
Ukrains'kyi biokhimichnyi zhurnal,
J Baardsnes, and
S Sidhu, and
A MacLeod, and
J Elliott, and
D Morden, and
J Watson, and
T Borgford
October 1965,
Biochimica et biophysica acta,
