In a mastitis control program based on eradication of intramammary infection, it is important to differentiate streptococci. According to earlier reports, Streptococcus dysgalactiae was unable to hydrolyze esculin. Preliminary data from the present study indicated that under optimal conditions, some strains of S dysgalactiae were capable of esculin hydrolysis. In a study of 45 strains of S dysgalactiae, 55.5% were able to hydrolyze esculin. In the past, these organisms would probably have been identified as Streptococcus uberis. Streptococcus dysgalactiae is more sensitive to antibiotics than S uberis, and the infection is easier to eradicate from infected glands.