A high-sucrose diet alters the lipid composition and fluidity of liver sinusoidal membranes. 1998

D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
Department of Pediatrics and Center for Human Nutrition, University of Colorado Health Sciences Center, Denver 80262, USA.

Impaired insulin suppression of hepatic glucose production and accumulation of hepatic triglycerides occur after 1 week on a high-sucrose diet. The purpose of this study was to ascertain whether changes in structural lipids, fatty acid composition and/or fluidity occur after 1 week on a high-sucrose diet, and therefore might contribute to the sucrose-induced impairment in hepatic glucose metabolism. Male Wistar rats (n=28) were fed a purified high starch (68% of energy) diet for a 2-week baseline period. Fourteen animals were then switched to a high sucrose (68% of energy) diet for 1 (n=7) or 5 (n=7) weeks. Analyses were performed on liver sinusoidal membranes (due to this membrane's involvement in nutrient transport) from overnight fasted rats. The degree of saturation of sinusoidal membrane phospholipids and liver triglyceride fatty acids was significantly greater in sucrose vs. starch at 1 and 5 weeks. This resulted in significantly lower sinusoidal membrane fluidity at 1 and 5 weeks in the sucrose group. In contrast, hepatic sinusoidal membrane cholesterol content (0.60+/-0.05 vs. 0.42+/-0.04 micromol/mg protein) and the cholesterol to phospholipid molar ratio (0.66+/-0.04 vs. 0.50+/-0.03) were significantly greater in sucrose vs. starch animals at 5 weeks only. Minimal differences were observed in individual phospholipid species between groups. These data suggest that changes in fatty acid composition and fluidity may contribute to the development of sucrose-induced hepatic insulin resistance.

UI MeSH Term Description Entries
D007425 Intracellular Membranes Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES. Membranes, Intracellular,Intracellular Membrane,Membrane, Intracellular
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008297 Male Males
D008560 Membrane Fluidity The motion of phospholipid molecules within the lipid bilayer, dependent on the classes of phospholipids present, their fatty acid composition and degree of unsaturation of the acyl chains, the cholesterol concentration, and temperature. Bilayer Fluidity,Bilayer Fluidities,Fluidities, Bilayer,Fluidities, Membrane,Fluidity, Bilayer,Fluidity, Membrane,Membrane Fluidities
D008563 Membrane Lipids Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation. Cell Membrane Lipid,Cell Membrane Lipids,Membrane Lipid,Lipid, Cell Membrane,Lipid, Membrane,Lipids, Cell Membrane,Lipids, Membrane,Membrane Lipid, Cell,Membrane Lipids, Cell
D010743 Phospholipids Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system. Phosphatides,Phospholipid
D001835 Body Weight The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms. Body Weights,Weight, Body,Weights, Body
D004734 Energy Metabolism The chemical reactions involved in the production and utilization of various forms of energy in cells. Bioenergetics,Energy Expenditure,Bioenergetic,Energy Expenditures,Energy Metabolisms,Expenditure, Energy,Expenditures, Energy,Metabolism, Energy,Metabolisms, Energy
D005227 Fatty Acids Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed) Aliphatic Acid,Esterified Fatty Acid,Fatty Acid,Fatty Acids, Esterified,Fatty Acids, Saturated,Saturated Fatty Acid,Aliphatic Acids,Acid, Aliphatic,Acid, Esterified Fatty,Acid, Saturated Fatty,Esterified Fatty Acids,Fatty Acid, Esterified,Fatty Acid, Saturated,Saturated Fatty Acids
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
May 1988, Biochemistry,
D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
January 1985, Biochimica et biophysica acta,
D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
March 1984, Biochemistry,
D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
May 1991, The Journal of experimental zoology,
D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
July 1988, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
February 1979, The Journal of nutrition,
D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
January 1989, Membrane biochemistry,
D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
August 1989, Journal of bioenergetics and biomembranes,
D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
February 1988, Biochimica et biophysica acta,
D A Podolin, and E Sutherland, and M Iwahashi, and F R Simon, and M J Pagliassotti
January 1987, Hepatology (Baltimore, Md.),
Copied contents to your clipboard!