BIOMAT Database Logo BIOMAT Database Logo

Unique 24-hydroxylated metabolites represent a significant pathway of metabolism of vitamin D2 in humans: 24-hydroxyvitamin D2 and 1,24-dihydroxyvitamin D2 detectable in human serum. 1998

E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
University Department of Medicine, Manchester Royal Infirmary, United Kingdom.

We have produced evidence for a new metabolic pathway for vitamin D2 in humans involving the production of 24-hydroxyvitamin D2 (24OHD2) and 1,24-dihydroxyvitamin D2 [1,24-(OH)2D2]. These metabolites were produced after either a single large dose (10(6) IU) of vitamin D2 or repeated daily doses between 10(3) and 5 x 10(4) IU. We developed assay systems for the metabolites in human serum and showed that in some chronically treated patients, the concentration of 1,24-(OH)2D2 equalled that of 1,25-(OH)2D2 at about 100 pmol/L. The metabolites were identified by high performance liquid chromatography with diode array spectrophotometry for 24OHD2 and by high resolution gas chromatography-mass spectrometry for 1,24-(OH)2D2. We show that 1,24-(OH)2D2 synthesis can be stimulated by PTH, indicating a renal origin for this metabolite and postulate that it is formed from 24OHD2, which may be synthesized in liver. We conclude from this study that vitamin D2 gives rise to two biologically active products, 1,24-(OH)2D2 and 1,25-(OH)2D2, and that 1,24-(OH)2D2 could be an attractive naturally occurring analog of 1,25-(OH)2D3 for clinical use.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008297 Male Males
D008401 Gas Chromatography-Mass Spectrometry A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. Chromatography, Gas-Liquid-Mass Spectrometry,Chromatography, Gas-Mass Spectrometry,GCMS,Spectrometry, Mass-Gas Chromatography,Spectrum Analysis, Mass-Gas Chromatography,Gas-Liquid Chromatography-Mass Spectrometry,Mass Spectrometry-Gas Chromatography,Chromatography, Gas Liquid Mass Spectrometry,Chromatography, Gas Mass Spectrometry,Chromatography, Mass Spectrometry-Gas,Chromatography-Mass Spectrometry, Gas,Chromatography-Mass Spectrometry, Gas-Liquid,Gas Chromatography Mass Spectrometry,Gas Liquid Chromatography Mass Spectrometry,Mass Spectrometry Gas Chromatography,Spectrometries, Mass-Gas Chromatography,Spectrometry, Gas Chromatography-Mass,Spectrometry, Gas-Liquid Chromatography-Mass,Spectrometry, Mass Gas Chromatography,Spectrometry-Gas Chromatography, Mass,Spectrum Analysis, Mass Gas Chromatography
D010281 Parathyroid Hormone A polypeptide hormone (84 amino acid residues) secreted by the PARATHYROID GLANDS which performs the essential role of maintaining intracellular CALCIUM levels in the body. Parathyroid hormone increases intracellular calcium by promoting the release of CALCIUM from BONE, increases the intestinal absorption of calcium, increases the renal tubular reabsorption of calcium, and increases the renal excretion of phosphates. Natpara,PTH (1-84),PTH(1-34),Parathormone,Parathyrin,Parathyroid Hormone (1-34),Parathyroid Hormone (1-84),Parathyroid Hormone Peptide (1-34),Hormone, Parathyroid
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004872 Ergocalciferols Derivatives of ERGOSTEROL formed by ULTRAVIOLET RAYS breaking of the C9-C10 bond. They differ from CHOLECALCIFEROL in having a double bond between C22 and C23 and a methyl group at C24. Calciferols,Vitamin D 2,Ergocalciferol,Vitamin D2,D2, Vitamin
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013058 Mass Spectrometry An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass

Related Publications

E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
Isolation and identification of 24-hydroxyvitamin D2 and 24,25-dihydroxyvitamin D2.
July 1980, Archives of biochemistry and biophysics,
E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
An alternative pathway of vitamin D metabolism. Cytochrome P450scc (CYP11A1)-mediated conversion to 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2.
July 2006, The FEBS journal,
E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
25-hydroxyvitamin D2, 25-hydroxyvitamin D3 and total 24,25-dihydroxyvitamin D in human serum.
February 1981, Annals of clinical research,
E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
24,26-Dihydroxyvitamin D2: a unique physiological metabolite of vitamin D2.
July 1988, Biochemistry,
E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
Evidence for the activation of 1alpha-hydroxyvitamin D2 by 25-hydroxyvitamin D-24-hydroxylase: delineation of pathways involving 1alpha,24-dihydroxyvitamin D2 and 1alpha,25-dihydroxyvitamin D2.
February 2006, Biochimica et biophysica acta,
E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
1,24(S)-dihydroxyvitamin D2, an endogenous vitamin D2 metabolite, inhibits growth of breast cancer cells and tumors.
January 2005, Anticancer research,
E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
Tissue distribution and activity studies of 1,24-dihydroxyvitamin D2, a metabolite of vitamin D2 with low calcemic activity in vivo.
October 2004, Biochemical pharmacology,
E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
Isolation and identification of 4,25-dihydroxyvitamin D2: a novel A-ring hydroxylated metabolite of vitamin D2.
November 1999, The Journal of steroid biochemistry and molecular biology,
E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
A pathway for the metabolism of vitamin D3: unique hydroxylated metabolites formed during catalysis with cytochrome P450scc (CYP11A1).
December 2003, Proceedings of the National Academy of Sciences of the United States of America,
E B Mawer, and G Jones, and M Davies, and P E Still, and V Byford, and N J Schroeder, and H L Makin, and C W Bishop, and J C Knutson
19-nor-10-ketovitamin D derivatives: unique metabolites of vitamin D3, vitamin D2, and 25-hydroxyvitamin D3.
July 1983, Biochemistry,
Copied contents to your clipboard!