-transparent.png){kind=logo}
1. The inhibition of the ADP-stimulated respiration of potato mitochondria by carboxyatractyloside is relieved by high concentration of ADP or by the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP). Atractyloside is a much less potent inhibitor than carboxyatractyloside. The inhibition of the ADP-stimulated respiration required about 60-times more atractyloside than carboxyatractyloside. 2. [35S]carboxyatractyloside and [3H]bongkrekic acid bind to potato mitochondria with high affinity (Kd = 10 to 20 nM, n=0.6-0.7 nmol per mg protein). Added ADP competes with carboxyatractyloside for binding; on the contrary ADP increases the amount of bound bongkrekic acid. [3H]atractyloside binds to potato mitochondria with a much lower affinity (Kd=0.45 muM) than carboxyatractyloside or bongkrekic acid. 3. Bound [3H]atractyloside is displaced by ADP, carboxyatractyloside and bongkrekic acid. The displacement of bound [35S]carboxyatractyloside by bongkrekic acid and of bound [3H]bongkrekic acid by carboxyatractyloside is markedly increased by ADP. 4. Bongkrekic acid competes with [35S]carboxyatractyloside for binding. Addition of a small concentration of ADP considerably enhances the inhibitory effect of bongkrekic acid on [35S]carboxyatractyloside binding. 5. The adenine nucleotide content of potato mitochondria is of the order of 1 nmol per mg protein. ADP transport in potato mitochondria is inhibited by atractyloside 30- to 40-times less efficiently than by carboxyatractyloside.
P V Vignais, and
R Douce, and
G J Lauquin, and
P M Vignais
January 1979,
Methods in enzymology,
P V Vignais, and
R Douce, and
G J Lauquin, and
P M Vignais
January 1975,
Biochemical Society transactions,
P V Vignais, and
R Douce, and
G J Lauquin, and
P M Vignais
September 1971,
FEBS letters,
P V Vignais, and
R Douce, and
G J Lauquin, and
P M Vignais
August 1975,
FEBS letters,
P V Vignais, and
R Douce, and
G J Lauquin, and
P M Vignais
April 2012,
Bioorganic & medicinal chemistry letters,
P V Vignais, and
R Douce, and
G J Lauquin, and
P M Vignais
February 1979,
FEBS letters,
P V Vignais, and
R Douce, and
G J Lauquin, and
P M Vignais
July 1974,
Biochemical and biophysical research communications,
P V Vignais, and
R Douce, and
G J Lauquin, and
P M Vignais
April 1965,
Biochimica et biophysica acta,
P V Vignais, and
R Douce, and
G J Lauquin, and
P M Vignais
August 1981,
FEBS letters,
P V Vignais, and
R Douce, and
G J Lauquin, and
P M Vignais
January 1979,
Methods in enzymology,
