Biomaterial Database

[Eserine-induced change in membrane potential of frog muscle fibers]. 1976

L G Mikaelian, and S A Adzhian

The action of eserine of acetylcholinesterase (AchE) reversible inhibitor of frog muscle fibres membrane potential (MP) under various physico-chemical conditions in external solution was studied. The data obtained show that changes of external pH in any direction decrease the depolarisation of the membrane produced by eserine. The dose-effect curve is linear at pH 7, but it has saturation at pH 6 and pH 9. Dependence of the membrane depolarisation in the presence of eserine upon calcium ions concentration in external solution is S-shape. Protonophore 3C1CCP (carbonilcianamid-m-3C1-phenylhydrazon) depolarises the membrane further in the presence of eserine. Valinomycin under these conditions completely restores the MP. Evidence is obtained that eserine reduces potassium permeability of the muscle membrane. It is supposed that membrane-bound AchE is involved in the ionic permeability regulation of the muscle membrane at rest.

UI	MeSH Term	Description	Entries
D008564	Membrane Potentials	The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).	Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D010830	Physostigmine	A cholinesterase inhibitor that is rapidly absorbed through membranes. It can be applied topically to the conjunctiva. It also can cross the blood-brain barrier and is used when central nervous system effects are desired, as in the treatment of severe anticholinergic toxicity.	Eserine
D011898	Ranidae	The family of true frogs of the order Anura. The family occurs worldwide except in Antarctica.	Frogs, True,Rana,Frog, True,True Frog,True Frogs
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002259	Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone	A proton ionophore that is commonly used as an uncoupling agent in biochemical studies.	Carbonyl Cyanide para-Trifluoromethoxyphenylhydrazone,FCCP,(4-(Trifluoromethoxy)phenyl)hydrazonopropanedinitrile,Carbonyl Cyanide p Trifluoromethoxyphenylhydrazone,Carbonyl Cyanide para Trifluoromethoxyphenylhydrazone,Cyanide p-Trifluoromethoxyphenylhydrazone, Carbonyl,Cyanide para-Trifluoromethoxyphenylhydrazone, Carbonyl,p-Trifluoromethoxyphenylhydrazone, Carbonyl Cyanide,para-Trifluoromethoxyphenylhydrazone, Carbonyl Cyanide
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014634	Valinomycin	A cyclododecadepsipeptide ionophore antibiotic produced by Streptomyces fulvissimus and related to the enniatins. It is composed of 3 moles each of L-valine, D-alpha-hydroxyisovaleric acid, D-valine, and L-lactic acid linked alternately to form a 36-membered ring. (From Merck Index, 11th ed) Valinomycin is a potassium selective ionophore and is commonly used as a tool in biochemical studies.
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In