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Methods for the generation of large numbers of different bispecific antibodies are presented. Cloning strategies are detailed to create repertoires of bispecific diabody molecules with variability on one or both of the antigen binding sites. This diabody format, when combined with the power of phage display technology, allows the generation and analysis of thousands of different bispecific molecules. Selection for binding presumably also selects for more stable diabodies. Phage diabody libraries enable screening or selection of the best combination bispecific molecule with regards to affinity of binding, epitope recognition and pairing before manufacture of the best candidate.
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G Walter, and
K FitzGerald, and
P Schuler, and
W Mahoney, and
A R Duncan, and
H R Hoogenboom
September 2016,
Biochemical and biophysical research communications,
B T McGuinness, and
G Walter, and
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P Schuler, and
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H R Hoogenboom
February 2002,
Journal of molecular biology,
B T McGuinness, and
G Walter, and
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January 2007,
Nature protocols,
B T McGuinness, and
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January 2018,
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B T McGuinness, and
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W Mahoney, and
A R Duncan, and
H R Hoogenboom
August 2003,
Journal of immunological methods,
B T McGuinness, and
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H R Hoogenboom
January 1997,
Cancer immunology, immunotherapy : CII,
B T McGuinness, and
G Walter, and
K FitzGerald, and
P Schuler, and
W Mahoney, and
A R Duncan, and
H R Hoogenboom
May 1993,
Nucleic acids research,
B T McGuinness, and
G Walter, and
K FitzGerald, and
P Schuler, and
W Mahoney, and
A R Duncan, and
H R Hoogenboom
January 2020,
Methods in molecular biology (Clifton, N.J.),
B T McGuinness, and
G Walter, and
K FitzGerald, and
P Schuler, and
W Mahoney, and
A R Duncan, and
H R Hoogenboom
January 2000,
Methods in enzymology,
B T McGuinness, and
G Walter, and
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P Schuler, and
W Mahoney, and
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H R Hoogenboom
August 2000,
Protein engineering,
