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In this study, the involvement of polymorphonuclear leukocytes (PMNs) in the development of intimal thickening was investigated. A fibromuscular intima was induced by placing a silicone collar around the rabbit carotid artery for 3 days or 2 weeks; the contralateral artery was sham operated. Rabbits received placebo treatments (groups 1 and 3), granulocyte-colony stimulating factor (group 2; G-CSF, 20 microg x kg(-1) x d(-1), delivered by subcutaneous osmotic pumps), or an anti-CD18 monoclonal antibody (group 4; 1.5 mg/kg i.v.). The G-CSF treatment raised the peripheral PMN count 5- to 12-fold but had no effect on intimal thickening on day 3, 12, or 14. A single injection of anti-CD18 prevented PMN extravasation 6 hours after collar implantation without influencing intimal hyperplasia on day 14. Repeated daily administration of anti-CD18 strongly bound to CD18 on peripheral PMNs and inhibited both PMN-dependent plasma extravasation in the skin and accumulation of CD14-immunoreactive leukocytes in the intima and media. However, anti-CD18 did not suppress early intimal thickening or accumulation of alpha-smooth muscle actin-immunoreactive cells by day 3. It thus appears that the PMN influx in the intima and media evoked by the perivascular collar is of little functional relevance to the subsequent smooth muscle cell migration and intimal thickening in this model.
D J Van Put, and
N Van Osselaer, and
G R De Meyer, and
L J Andries, and
M M Kockx, and
L S De Clerck, and
H Bult
April 2002,
Experimental cell research,
D J Van Put, and
N Van Osselaer, and
G R De Meyer, and
L J Andries, and
M M Kockx, and
L S De Clerck, and
H Bult
November 1994,
Arteriosclerosis and thrombosis : a journal of vascular biology,
D J Van Put, and
N Van Osselaer, and
G R De Meyer, and
L J Andries, and
M M Kockx, and
L S De Clerck, and
H Bult
February 1997,
Atherosclerosis,
D J Van Put, and
N Van Osselaer, and
G R De Meyer, and
L J Andries, and
M M Kockx, and
L S De Clerck, and
H Bult
December 1998,
International journal of cardiology,
D J Van Put, and
N Van Osselaer, and
G R De Meyer, and
L J Andries, and
M M Kockx, and
L S De Clerck, and
H Bult
April 1999,
The Journal of pharmacy and pharmacology,
D J Van Put, and
N Van Osselaer, and
G R De Meyer, and
L J Andries, and
M M Kockx, and
L S De Clerck, and
H Bult
March 2012,
Molecular medicine reports,
D J Van Put, and
N Van Osselaer, and
G R De Meyer, and
L J Andries, and
M M Kockx, and
L S De Clerck, and
H Bult
November 1999,
Arteriosclerosis, thrombosis, and vascular biology,
D J Van Put, and
N Van Osselaer, and
G R De Meyer, and
L J Andries, and
M M Kockx, and
L S De Clerck, and
H Bult
January 2015,
Drug design, development and therapy,
D J Van Put, and
N Van Osselaer, and
G R De Meyer, and
L J Andries, and
M M Kockx, and
L S De Clerck, and
H Bult
October 1995,
Journal of cardiovascular pharmacology,
D J Van Put, and
N Van Osselaer, and
G R De Meyer, and
L J Andries, and
M M Kockx, and
L S De Clerck, and
H Bult
January 2001,
Pharmacological research,
