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BACKGROUND Allogeneic blood transfusion is associated with an increased risk of infection and higher cancer recurrence rates. Previous research has shown that blood transfusion results in multiple immune effects, including cytokine alterations. The purpose of this study was to measure the long term kinetics of splenocyte cytokine production in transfused mice. METHODS Balb/c mice received either syngeneic transfusion (Syn-BT) or allogeneic transfusion (Allo-BT) from C3H-HeN mice. Splenocyte production of IL-2, IL-6, IL-10, and IFN-gamma was quantitated by ELISA on post-transfusion days 5, 10, 21, and 30. RESULTS Both Allo-BT and Syn-BT produced significant alterations in cytokine production, but Allo-BT produced the most dramatic and enduring effects as summarized: IL-2: Production of IL-2 was suppressed at day 5, (p < 0.0001), but then rose, peaking at day 21, 30% greater than control values (p < 0.05). IL-6: Allo-BT mice showed suppression of IL-6 throughout the study period (p < 0.005 vs controls, each time point). IL-10: A 5-fold increase in IL-10 production was seen at day 5 after Allo-BT (p < 0.0001 vs control). Production of IL-10 was suppressed at days 10 and 21 (p < 0.001), but returned to control levels by day 30, gamma-IFN: At day 5 post Allo-BT, gamma-IFN was 4 x greater than controls (p < 0.0001). Gamma-IFN production was suppressed at day 10, but then rose at days 21 and 30 to nearly 3 x control levels (p < 0.0001). CONCLUSIONS Allo-BT produced multiple cytokine alterations that were of prolonged duration. These results provide a theoretic explanation for the multiple, long-term immunomodulating effects seen in patients who have received transfusions.
W Shao, and
L S Edelman, and
D J Sullivan, and
E W Nelson, and
J Shelby
August 2023,
Anesthesia and analgesia,
W Shao, and
L S Edelman, and
D J Sullivan, and
E W Nelson, and
J Shelby
February 1995,
Lancet (London, England),
W Shao, and
L S Edelman, and
D J Sullivan, and
E W Nelson, and
J Shelby
April 2004,
Clinical and laboratory haematology,
W Shao, and
L S Edelman, and
D J Sullivan, and
E W Nelson, and
J Shelby
February 2006,
Clinical oncology (Royal College of Radiologists (Great Britain)),
W Shao, and
L S Edelman, and
D J Sullivan, and
E W Nelson, and
J Shelby
August 1982,
Surgery,
W Shao, and
L S Edelman, and
D J Sullivan, and
E W Nelson, and
J Shelby
June 1994,
Transfusion,
W Shao, and
L S Edelman, and
D J Sullivan, and
E W Nelson, and
J Shelby
April 1974,
The American journal of medicine,
W Shao, and
L S Edelman, and
D J Sullivan, and
E W Nelson, and
J Shelby
January 1994,
Vox sanguinis,
W Shao, and
L S Edelman, and
D J Sullivan, and
E W Nelson, and
J Shelby
June 2001,
The Journal of bone and joint surgery. American volume,
W Shao, and
L S Edelman, and
D J Sullivan, and
E W Nelson, and
J Shelby
November 2004,
Cancer,
