Biomaterial Database

Ultrastructural localization of mu-1 opioid receptor in the dorsal raphe nucleus of the rat. 1998

Q P Wang, and H Ochiai, and J L Guan, and Y Nakai

Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.

A simple pre-embedding avidin-biotin-peroxidase complex technique was used to study the ultrastructural localization of mu-1 opioid receptor in the rat dorsal raphe nucleus. Using low concentrations of the first antiserum for incubation with a short reaction time to 3,3'-diaminobenzidine, the immunostaining was faint at the light microscopic level. However, at the electron microscopic level strong immunoreaction was observed. Mu-1 opioid receptors were found to be localized on the postsynaptic membrane of dendrites, extra-synaptic plasma membrane, and the surface of the small, clear vesicles in axon terminals. Of the total 407 immunopositive profiles observed, 76.4% (311/407) were dendrites and 18.9% (77/407) were axon terminals. The immunostained myelinated axons and perikarya were relatively rare, with frequencies of 1.0% (4/407) and 3.7% (15/407), respectively. About 50.8% of the immunopositive dendrites (158/311) were immunostained having their MOR-LI results beneath the postsynaptic membrane, although about 19.6% of them (31/158) also exhibited MOR-LI on other components, including the extrasynaptic plasma membrane. Other immunopositive dendrites showed staining in some other contents, including extrasynaptic plasma membrane (82/311, 26.4%) or not on the plasma membranes (71/311, 22.8%). Less than half of the immunopositive axon terminals (35/77, 45.5%) were found to make synapses with nonimmunoreactive dendrites (31/77, 40.3%) or immunopositive dendrites (4/77, 5.2%); none were found to make synapses with immunoreactive perikarya. The present study shows that mu-1 opioid receptor in the dorsal raphe nucleus plays a role at both synapse or not.

UI	MeSH Term	Description	Entries
D007158	Immunologic Techniques	Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.	Antibody Dissociation,Immunologic Technic,Immunologic Technics,Immunologic Technique,Immunological Technics,Immunological Techniques,Technic, Immunologic,Technics, Immunologic,Technique, Immunologic,Techniques, Immunologic,Antibody Dissociations,Dissociation, Antibody,Dissociations, Antibody,Immunological Technic,Immunological Technique,Technic, Immunological,Technics, Immunological,Technique, Immunological,Techniques, Immunological
D008297	Male		Males
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D011903	Raphe Nuclei	Collections of small neurons centrally scattered among many fibers from the level of the TROCHLEAR NUCLEUS in the midbrain to the hypoglossal area in the MEDULLA OBLONGATA.	Caudal Linear Nucleus of the Raphe,Interfascicular Nucleus,Nucleus Incertus,Rostral Linear Nucleus of Raphe,Rostral Linear Nucleus of the Raphe,Superior Central Nucleus,Central Nucleus, Superior,Incertus, Nucleus,Nuclei, Raphe,Nucleus, Interfascicular,Nucleus, Raphe,Nucleus, Superior Central,Raphe Nucleus
D003712	Dendrites	Extensions of the nerve cell body. They are short and branched and receive stimuli from other NEURONS.	Dendrite
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013194	Staining and Labeling	The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.	Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings
D013569	Synapses	Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions.	Synapse
D014018	Tissue Distribution	Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.	Distribution, Tissue,Distributions, Tissue,Tissue Distributions
D017208	Rats, Wistar	A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.	Wistar Rat,Rat, Wistar,Wistar Rats