In earlier studies, we found that a late gene product, glycoprotein B (gB) was highly expressed in lymphoid tissues of chickens inoculated with turkey herpesvirus (HVT). The objectives of the present study were twofold. First, we wanted to expand on our previous research and determine if gB expression declines or disappears during later time periods of HVT infection. Second, we wanted to correlate gB expression with presence of HVT, i.e. if gB expression is absent, can HVT still be detected? Fifteen 1-day-old chicks were inoculated by intraperitoneal inoculation with 2000 plaque forming units of strain FC126 HVT. Thymus, spleen, bursa, brachial plexus, sciatic plexus, and feather tips were harvested at 21, 28, 35, 70, and 105 days postinoculation (PI). Brachial plexus and sciatic plexus were examined at 21, 28, and 35 days PI, and feather tips were examined at 21 and 28 days PI. An indirect immunofluorescence assay was used to detect HVT gB expression, and an in situ hybridization assay was used to detect HVT. At 21 days PI, gB expression was present in the thymus, spleen, and bursa. At 28 and 35 days PI, gB expression was detected in the thymus and spleen. At 70 days PI, gB expression was detected only in the spleen, and at 105 days PI, gB expression was not detected in any of the lymphoid tissue (thymus, spleen, or bursa). gB expression was not detected in the brachial plexus, sciatic plexus, or feather tips at any of the five time points. The bursa contained HVT only at 21 and 28 days PI. However, HVT was demonstrated in all other tissues from 21 to 105 days PI. Progression from a productive HVT infection to a latent HVT infection results in the loss of gB expression. Throughout this progression, a region of the HVT genome can be detected by appropriate methods.