Biomaterial Database

Ultrastructural determination of the early stage of conversion from root primordia to shoot primordia in vitro cultures of Solanum lycopersicoides Dun. 1997

A Tylicki, and W Burza, and M Kuraś, and S Malepszy

Department of Plant Morphogenesis, University of Warsaw.

The object of the research were in vitro cultures of root primordia and the early stage of conversion from root to shoot primordia cultures (ESCRS) of Solanum lycopersicoides Dun. The cell ultrastructure of both culture types was studied in order to determine processes underlying the root and shoot morphogenesis type. It was found that a change of a morphogenesis type occurs together with reorganization of aggregate structure and the cell ultrastructural organization. There were 4 zones detected in root primordia culture aggregates (the surface, the starch, the division and the differentiation zone) and 3 zones in ESCRS culture aggregates (the surface, the inner and the differentiation zone). The differences were observed also in storage substances accumulated in a culture (starch in root primordia cultures; proteins, lipids and starch in ESCRS ones).

UI	MeSH Term	Description	Entries
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D018517	Plant Roots	The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)	Plant Bulbs,Plant Root,Bulb, Plant,Bulbs, Plant,Plant Bulb,Root, Plant,Roots, Plant
D018520	Plant Shoots	New immature growth of a plant including stem, leaves, tips of branches, and SEEDLINGS.	Plant Shoot,Shoot, Plant,Shoots, Plant
D019657	Solanaceae	A plant family of the order SOLANALES, class MAGNOLIOPSIDA. Among the most noted are POTATOES; TOMATOES; CAPSICUM (green and red peppers); TOBACCO; and BELLADONNA.	Acnistus,Dunalia,Dunalias