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We have examined the question, is the carbohydrate moiety of ovalbumin attached to nascent chains or to the completed protein after its release from polyribosomes. Ovalbumin nascent chains have been isolated from hen oviduct by immunoprecipitation of ovalbumin-synthesizing polysomes and subsequent isolation of peptidyl-tRNA by DEAE-cellulose chromatography. Our results indicate that both glucosamine and mannose are incorporated into ovalbumin nascent chains by hen oviduct fragments. Although the asparagine to which the carbohydrates are attached is 10 amino acid residues from the NH2 terminus, the majority of the carbohydrate, if not all, is attached only to those peptide chains that have been essentially completed.
M L Kiely, and
G S McKnight, and
R T Schimke
May 1976,
Journal of biochemistry,
M L Kiely, and
G S McKnight, and
R T Schimke
January 1976,
Journal of steroid biochemistry,
M L Kiely, and
G S McKnight, and
R T Schimke
October 1976,
Archives of biochemistry and biophysics,
M L Kiely, and
G S McKnight, and
R T Schimke
October 1987,
Nucleic acids research,
M L Kiely, and
G S McKnight, and
R T Schimke
February 1987,
Zentralblatt fur Veterinarmedizin. Reihe A,
M L Kiely, and
G S McKnight, and
R T Schimke
January 1975,
Biochemistry,
M L Kiely, and
G S McKnight, and
R T Schimke
July 1984,
Carbohydrate research,
M L Kiely, and
G S McKnight, and
R T Schimke
May 1968,
Monatshefte fur Veterinarmedizin,
M L Kiely, and
G S McKnight, and
R T Schimke
October 1980,
The Journal of biological chemistry,
M L Kiely, and
G S McKnight, and
R T Schimke
September 1987,
European journal of biochemistry,
