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Endothelin-3 (ET-3), a potent vasoactive peptide, is considered to be produced from big ET-3 by endothelin-converting enzyme (ECE) like the other members of the endothelin family (ET-1 and ET-2). We purified a novel ECE from bovine iris microsomes. The purified enzyme, a 140 kDa protein by SDS-PAGE analysis, converted big ET-3 to ET-3 but not big ET-1, with a Km value of 0.14 microM for big ET-3. The conversion to ET-3 was confirmed with sandwich EIA by monoclonal antibodies, the elution profile of HPLC, and intracellular calcium mobilization in CHO-K1 cells expressing recombinant human ET(B) receptors. The conversion activity was inhibited by an inhibitor of neutral endopeptidase 24.11 (NEP) phosphoramidon. These results show that ECE-3 purified from bovine iris is a novel metalloprotease totally different from ECE-1 or ECE-2, in that the enzyme is highly specific for big ET-3.
H Hasegawa, and
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J Kimura, and
T Masaki
January 2000,
British journal of pharmacology,
H Hasegawa, and
K Hiki, and
T Sawamura, and
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T Masaki
December 1993,
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H Hasegawa, and
K Hiki, and
T Sawamura, and
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T Masaki
January 1993,
Life sciences,
H Hasegawa, and
K Hiki, and
T Sawamura, and
T Aoyama, and
Y Okamoto, and
S Miwa, and
S Shimohama, and
J Kimura, and
T Masaki
October 1991,
Naunyn-Schmiedeberg's archives of pharmacology,
H Hasegawa, and
K Hiki, and
T Sawamura, and
T Aoyama, and
Y Okamoto, and
S Miwa, and
S Shimohama, and
J Kimura, and
T Masaki
June 1992,
FEBS letters,
H Hasegawa, and
K Hiki, and
T Sawamura, and
T Aoyama, and
Y Okamoto, and
S Miwa, and
S Shimohama, and
J Kimura, and
T Masaki
January 1998,
Journal of cardiovascular pharmacology,
H Hasegawa, and
K Hiki, and
T Sawamura, and
T Aoyama, and
Y Okamoto, and
S Miwa, and
S Shimohama, and
J Kimura, and
T Masaki
October 1991,
Biochemical and biophysical research communications,
H Hasegawa, and
K Hiki, and
T Sawamura, and
T Aoyama, and
Y Okamoto, and
S Miwa, and
S Shimohama, and
J Kimura, and
T Masaki
January 1993,
Journal of cardiovascular pharmacology,
H Hasegawa, and
K Hiki, and
T Sawamura, and
T Aoyama, and
Y Okamoto, and
S Miwa, and
S Shimohama, and
J Kimura, and
T Masaki
February 1999,
Biochemistry,
H Hasegawa, and
K Hiki, and
T Sawamura, and
T Aoyama, and
Y Okamoto, and
S Miwa, and
S Shimohama, and
J Kimura, and
T Masaki
January 1998,
Journal of cardiovascular pharmacology,
