Biomaterial Database

Silicic acid thin-layer chromatography of conjugated and free bile acids. 1976

M N Chavez, and C L Krone

The separation of free bile acids, taurine conjugates, and glycine conjugates from one another was achieved by thin-layer chromatography, using a solvent system of isopropanol glacial acetic acid 93:7 in a solvent system of hexane-methylethylketone-glacial acetic acid 56:36:8 (v/v), it was possible to separate cholic, chenodeoxycholic, deoxycholic, and lithocholic acids. Application to a biological bile sample was demonstrated.

UI	MeSH Term	Description	Entries
D002855	Chromatography, Thin Layer	Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D001647	Bile Acids and Salts	Steroid acids and salts. The primary bile acids are derived from cholesterol in the liver and usually conjugated with glycine or taurine. The secondary bile acids are further modified by bacteria in the intestine. They play an important role in the digestion and absorption of fat. They have also been used pharmacologically, especially in the treatment of gallstones.	Bile Acid,Bile Salt,Bile Salts,Bile Acids,Acid, Bile,Acids, Bile,Salt, Bile,Salts, Bile
D012824	Silicic Acid	A hydrated form of silicon dioxide. It is commonly used in the manufacture of TOOTHPASTES and as a stationary phase for CHROMATOGRAPHY.	Hydrated Silica Gel,Silica Hydrate,Silicate Hydrate,Gel, Hydrated Silica,Hydrate, Silica,Hydrate, Silicate,Silica Gel, Hydrated