-transparent.png){kind=logo}
Neither Sequenase 2.0 nor Klenow fragment were able to extend 12-mer primers using the eight templates (16-mers) derived by placing each of the four isomeric benzo[a]pyrene diol epoxide-deoxyguanosine adducts at the 13th nucleotide from the 3'-end of two different sequence contexts. Using an 11-mer primer to get a running start did not overcome the adduct induced block of primer extension except for the Klenow fragment and one of the two sequence contexts, indicating primer extension is dependent on both the polymerase and sequence context. In this case, purine nucleoside triphosphates (dATP>dGTP) were incorporated opposite each of the four adducts.
Related Publications
L J Lipinski, and
H L Ross, and
B Zajc, and
J M Sayer, and
D M Jerina, and
A Dipple
October 2002,
Mutation research,
L J Lipinski, and
H L Ross, and
B Zajc, and
J M Sayer, and
D M Jerina, and
A Dipple
December 1988,
Proceedings of the National Academy of Sciences of the United States of America,
L J Lipinski, and
H L Ross, and
B Zajc, and
J M Sayer, and
D M Jerina, and
A Dipple
June 1985,
Japanese journal of cancer research : Gann,
L J Lipinski, and
H L Ross, and
B Zajc, and
J M Sayer, and
D M Jerina, and
A Dipple
June 1982,
Mutation research,
L J Lipinski, and
H L Ross, and
B Zajc, and
J M Sayer, and
D M Jerina, and
A Dipple
October 1977,
Nature,
L J Lipinski, and
H L Ross, and
B Zajc, and
J M Sayer, and
D M Jerina, and
A Dipple
December 2002,
Nucleic acids research,
L J Lipinski, and
H L Ross, and
B Zajc, and
J M Sayer, and
D M Jerina, and
A Dipple
January 2004,
Nucleic acids research,
L J Lipinski, and
H L Ross, and
B Zajc, and
J M Sayer, and
D M Jerina, and
A Dipple
February 2002,
The Journal of biological chemistry,
L J Lipinski, and
H L Ross, and
B Zajc, and
J M Sayer, and
D M Jerina, and
A Dipple
December 1977,
Proceedings of the National Academy of Sciences of the United States of America,
L J Lipinski, and
H L Ross, and
B Zajc, and
J M Sayer, and
D M Jerina, and
A Dipple
April 2002,
The Journal of biological chemistry,
