Biomaterial Database

A semisynthetic fetal calf serum-free liquid medium for in vitro cultivation of Leishmania promastigotes. 1998

S A Ali, and J Iqbal, and B Ahmad, and M Masoom

Institute of Biochemistry, and Department of Pharmacy, University of Balochistan, Quetta, Pakistan.

A semisynthetic, autoclavable, liquid medium that does not require inclusion of fetal calf serum (FCS) for the successful cultivation of Leishmania promastigotes in vitro is described. The parasites were grown in commercially available medium 199 supplemented with 10% FCS and 2% mammalian urine and the growth was compared with the same medium without FCS. The growth of the parasites in the semidefined medium without FCS was found to be as good as or slightly better that with medium 199 supplemented with FCS.

UI	MeSH Term	Description	Entries
D007891	Leishmania	A genus of flagellate protozoa comprising several species that are pathogenic for humans. Organisms of this genus have an amastigote and a promastigote stage in their life cycles. As a result of enzymatic studies this single genus has been divided into two subgenera: Leishmania leishmania and Leishmania viannia. Species within the Leishmania leishmania subgenus include: L. aethiopica, L. arabica, L. donovani, L. enrietti, L. gerbilli, L. hertigi, L. infantum, L. major, L. mexicana, and L. tropica. The following species are those that compose the Leishmania viannia subgenus: L. braziliensis, L. guyanensis, L. lainsoni, L. naiffi, and L. shawi.	Leishmania (Leishmania),Leishmania (Viannia),Leishmania leishmania,Leishmania viannia,Leishmania leishmanias,Leishmania viannias,Leishmanias,Leishmanias (Leishmania),Leishmanias (Viannia),leishmanias, Leishmania,viannias, Leishmania
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013242	Sterilization	The destroying of all forms of life, especially microorganisms, by heat, chemical, or other means.
D016895	Culture Media, Serum-Free	CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.	Protein-Free Media,Serum-Free Media,Low-Serum Media,Culture Media, Serum Free,Low Serum Media,Media, Low-Serum,Media, Protein-Free,Media, Serum-Free,Media, Serum-Free Culture,Protein Free Media,Serum Free Media,Serum-Free Culture Media
D018320	Leishmania major	A parasitic hemoflagellate of the subgenus Leishmania leishmania that infects man and animals and causes cutaneous leishmaniasis (LEISHMANIASIS, CUTANEOUS) of the Old World. Transmission is by Phlebotomus sandflies.	Leishmania (Leishmania) major,Leishmania tropica major,Leishmania leishmania major,Leishmania leishmania majors,Leishmania majors,Leishmania tropica majors,leishmania major, Leishmania,major, Leishmania leishmania,majors, Leishmania,majors, Leishmania leishmania,majors, Leishmania tropica,tropica major, Leishmania,tropica majors, Leishmania