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OBJECTIVE Adeno-associated virus (AAV) is emerging as a promising vector for gene therapy METHODS To determine the ability of recombinant AAV (rAAV) to express and integrate exogenous DNA into human retinal pigment epithelium (RPE) cells, a rAAV-GFP vector containing the green fluorescent protein (gfp) and neomycin resistance (neo(r)) genes was constructed and used to transduce RPE 407A cell line. RESULTS Fluorescent RPE cell clones were obtained and were confirmed to still be expressing GFP after 24 passages (3.5 months). CONCLUSIONS Adeno-associated virus-based vectors are able to efficiently transduce and stably persist in RPE cells.
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