Culture and characterization of smooth muscle cells from porcine retinal microvessels. 1998

B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
Lions Eye Institute and Centre for Ophthalmology and Visual Science, The University of Western Australia, Nedlands, Australia. mortimer@cyllene.uwa.edu.au

OBJECTIVE To establish long-term cultures of retinal vascular smooth muscle cells for future studies of their functions under normal and diseased conditions. METHODS Smooth muscle cells (SMC) from porcine retinal microvessels were isolated and cultured. RESULTS Cells in culture retained the common biochemical characteristics of SMC propagated from other sources. The cells were large, polygonal, spindle shaped and demonstrated the presence of smooth muscle-specific alpha-actin. Post-confluent cultures showed the 'hill and valley' multilayer growth characteristics. However, the growth rate was lower and the population doubling time was significantly larger than those reported for SMC propagated from big vessels. CONCLUSIONS Retinal vascular SMC could be cultured separately from other cell types. The availability of this culture provides a unique model for functional and metabolic studies of the retinal vessels.

UI MeSH Term Description Entries
D008841 Actin Cytoskeleton Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments. Actin Filaments,Microfilaments,Actin Microfilaments,Actin Cytoskeletons,Actin Filament,Actin Microfilament,Cytoskeleton, Actin,Cytoskeletons, Actin,Filament, Actin,Filaments, Actin,Microfilament,Microfilament, Actin,Microfilaments, Actin
D009131 Muscle, Smooth, Vascular The nonstriated involuntary muscle tissue of blood vessels. Vascular Smooth Muscle,Muscle, Vascular Smooth,Muscles, Vascular Smooth,Smooth Muscle, Vascular,Smooth Muscles, Vascular,Vascular Smooth Muscles
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D000199 Actins Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle. F-Actin,G-Actin,Actin,Isoactin,N-Actin,alpha-Actin,alpha-Isoactin,beta-Actin,gamma-Actin,F Actin,G Actin,N Actin,alpha Actin,alpha Isoactin,beta Actin,gamma Actin
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D012171 Retinal Vessels The blood vessels which supply and drain the RETINA. Pecten Oculi,Retinal Vasculature,Retinal Blood Vessels,Retinal Blood Vessel,Retinal Vasculatures,Retinal Vessel,Vasculature, Retinal,Vessel, Retinal,Vessel, Retinal Blood
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D018929 Cell Culture Techniques Methods for maintaining or growing CELLS in vitro. Cell Culture,Cell Culture Technique,Cell Cultures,Culture Technique, Cell,Culture Techniques, Cell

Related Publications

B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
September 1991, Cell and tissue research,
B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
January 2012, Methods in molecular biology (Clifton, N.J.),
B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
January 2001, Methods in molecular medicine,
B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
January 2016, Methods in molecular biology (Clifton, N.J.),
B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
February 2011, Acta neurochirurgica,
B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
October 2020, Tissue & cell,
B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
March 2004, The Journal of urology,
B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
September 1992, The American journal of physiology,
B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
January 2003, Methods in molecular medicine,
B C Mortimer, and D Y Yu, and W H Morgan, and V A Alder
March 1999, In vitro cellular & developmental biology. Animal,
Copied contents to your clipboard!