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64 Haemophilus influenzae strains circulating in Havana City during a year were characterized by the carbohydrate fermentation method for the first time in Cuba. The fermentative pattern D was the most frequently found. Patterns D and G together were 72% of the total of strains studied. The combination of the carbohydrate fermentation with serotyping and biotyping allowed a greater differentiation of strains (14 groups). Patterns A, B, C and F appeared in children over 6 months of age, and pattern G in the group from 6 to 18. Patterns D and G predominated in the bacterial meningoencephalitis. A higher heterogeneity was observed among the strains isolated from acute respiratory infections. Some of the advantages of the Haemophilus influenzae strains subtyping method are stressed, such as: simplicity, easiness to be applied and interpreted, and the fact that it is not necessary a qualified personnel or a specialized laboratory for its implementation.
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