BIOMAT Database Logo BIOMAT Database Logo

Differentiation of adult Leydig cells in the neonatal rat testis is arrested by hypothyroidism. 1998

S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
Department of Animal Science, College of Veterinary Medicine, The University of Tennessee, Knoxville, Tennessee 37996, USA. mendisc@utk.edu

The effects of propyl thiouracil (PTU)-induced hypothyroidism on testicular interstitial cells and androgen secretion in vitro in the neonatal rat were investigated using Sprague Dawley rats of 1, 7, 14, and 21 days. The results revealed that the fetal Leydig cell (FLC) number per testis was unchanged between and within treatment groups at all ages tested. FLC size was 50% smaller in 21-day controls than in all other groups. Adult Leydig cells (ALCs) were present at Days 14 and 21 in controls but were absent in PTU rats. ALCs approximated FLCs of 21-day controls in size. ALC number per testis showed a sharp increase at Day 21. 11ss-HSD1-positive cells were absent in 21-day PTU testes, but a few were present in 21-day control testes. Testosterone secretion per testis was unchanged in 1- to 21-day controls and 7- to 21-day PTU rats. However, at Day 21, a significantly lower value was seen in controls compared to PTU rats. Testicular androstenedione secretion was not significantly different between control and PTU rats up to 14 days, but a sharp rise was observed in controls at Day 21. At this age, androstenedione levels in PTU rats were similar to those at younger ages. In summary, histological studies showed that hypothyroidism prevented the hypotrophy of FLC and the emergence of ALC in the neonatal rat testis, and agreed favorably with results concerning testicular androgen secretion in vitro. These findings suggest that thyroid hormones have a regulatory role in precursor cell differentiation into Leydig cells in the neonatal rat testis to establish the ALC population.

UI MeSH Term Description Entries
D007037 Hypothyroidism A syndrome that results from abnormally low secretion of THYROID HORMONES from the THYROID GLAND, leading to a decrease in BASAL METABOLIC RATE. In its most severe form, there is accumulation of MUCOPOLYSACCHARIDES in the SKIN and EDEMA, known as MYXEDEMA. It may be primary or secondary due to other pituitary disease, or hypothalamic dysfunction. Central Hypothyroidism,Primary Hypothyroidism,Secondary Hypothyroidism,TSH Deficiency,Thyroid-Stimulating Hormone Deficiency,Central Hypothyroidisms,Deficiency, TSH,Deficiency, Thyroid-Stimulating Hormone,Hormone Deficiency, Thyroid-Stimulating,Hypothyroidism, Central,Hypothyroidism, Primary,Hypothyroidism, Secondary,Hypothyroidisms,Primary Hypothyroidisms,Secondary Hypothyroidisms,TSH Deficiencies,Thyroid Stimulating Hormone Deficiency,Thyroid-Stimulating Hormone Deficiencies
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007985 Leydig Cells Steroid-producing cells in the interstitial tissue of the TESTIS. They are under the regulation of PITUITARY HORMONES; LUTEINIZING HORMONE; or interstitial cell-stimulating hormone. TESTOSTERONE is the major androgen (ANDROGENS) produced. Interstitial Cells, Testicular,Leydig Cell,Testicular Interstitial Cell,Testicular Interstitial Cells,Cell, Leydig,Cell, Testicular Interstitial,Cells, Leydig,Cells, Testicular Interstitial,Interstitial Cell, Testicular
D008297 Male Males
D009929 Organ Size The measurement of an organ in volume, mass, or heaviness. Organ Volume,Organ Weight,Size, Organ,Weight, Organ
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D011441 Propylthiouracil A thiourea antithyroid agent. Propythiouracil inhibits the synthesis of thyroxine and inhibits the peripheral conversion of throxine to tri-iodothyronine. It is used in the treatment of hyperthyroidism. (From Martindale, The Extra Pharmacopeoia, 30th ed, p534) 6-Propyl-2-Thiouracil,6 Propyl 2 Thiouracil
D001835 Body Weight The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms. Body Weights,Weight, Body,Weights, Body
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D005260 Female Females

Related Publications

S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
Increased proliferation of Leydig cells induced by neonatal hypothyroidism in the rat.
January 1996, Journal of andrology,
S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
Leydig cells increase their numbers but decline in steroidogenic function in the adult rat after neonatal hypothyroidism.
June 1993, Endocrinology,
S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
Calretinin is expressed in the Leydig cells of rat testis.
October 1994, Biochimica et biophysica acta,
S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
The increased number of Leydig cells by di(2-ethylhexyl) phthalate comes from the differentiation of stem cells into Leydig cell lineage in the adult rat testis.
April 2013, Toxicology,
S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
Experimental control of the differentiation of Leydig cells in the rat fetal testis.
November 1988, Proceedings of the National Academy of Sciences of the United States of America,
S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
Regulation of the proliferation and differentiation of Leydig stem cells in the adult testis.
June 2014, Biology of reproduction,
S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
Postnatal differentiation of Leydig cells in the rabbit testis.
February 1976, The American journal of anatomy,
S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
Differentiation of adult Leydig cells.
June 1995, The Journal of steroid biochemistry and molecular biology,
S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
Morphological studies on the origin of adult-type Leydig cells in rat testis.
January 1995, Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia,
S M Mendis-Handagama, and H B Ariyaratne, and K R Teunissen van Manen, and R L Haupt
Functional maturation of rat testis Leydig cells.
January 1984, Annals of the New York Academy of Sciences,
Copied contents to your clipboard!