BIOMAT Database Logo BIOMAT Database Logo

Viruses produced from complementary DNA of virulent and avirulent strains of swine vesicular disease viruses retain the in vivo and in vitro characteristics of the parental strain. 1998

T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
Department of Exotic Disease, National Institute of Animal Health, Tokyo, Japan.

A full-length cDNA copy of the genome of the pathogenic strain, J1'73, of swine vesicular disease virus (SVDV) was constructed and inserted into the plasmid pSVL to generate a recombinant plasmid pSVLSJ1. Infectious virus was produced following transfection of cultured mammalian cells with the plasmid. The recovered virus had the same in vitro properties as the parental strain with regard to antigenicity, plaque size on IBRS-2 cells and single-step growth. Pigs were experimentally infected with the parental virus, J1'73 strain, and viruses recovered from cells transfected with the plasmids pSVLSJ1 and pSVLS00 [Inoue T, Yamaguchi S, Saeki T, Sekiguchi K, J Gen Virol 71: 1,835-1,838 (1990)] corresponding to the pathogenic (J1'73) and non-pathogenic (H/3'76) Japanese strains of the SVDV, respectively. All pigs inoculated with the virus recovered from pSVLSJ1 produced clinical signs of similar severity to those inoculated with the parental J1'73 strain. In contrast, pigs inoculated with the virus recovered from pSVLS00 did not show any clinical signs. Viruses recovered from cells transfected with either pSVLSJ1 or pSVLS00 therefore retained the in vitro characteristics and the in vivo pathogenicity of their respective parental strains.

UI MeSH Term Description Entries
D004770 Enterovirus A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus". Coxsackie Viruses,Coxsackieviruses
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013555 Swine Vesicular Disease An enterovirus infection of swine clinically indistinguishable from FOOT-AND-MOUTH DISEASE, vesicular stomatitis, and VESICULAR EXANTHEMA OF SWINE. It is caused by a strain of HUMAN ENTEROVIRUS B. Swine Vesicular Diseases,Vesicular Disease, Swine,Vesicular Diseases, Swine
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D014766 Viremia The presence of viruses in the blood. Viremias
D014774 Virulence The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS. Pathogenicity
D018076 DNA, Complementary Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe. Complementary DNA,cDNA,cDNA Probes,Probes, cDNA
D019556 COS Cells CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CHLOROCEBUS AETHIOPS).) COS-1 Cells,COS-7 Cells,COS 1 Cells,COS 7 Cells,COS Cell,COS-1 Cell,COS-7 Cell,Cell, COS,Cell, COS-1,Cell, COS-7,Cells, COS,Cells, COS-1,Cells, COS-7

Related Publications

T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain.
January 2006, Chinese science bulletin = Kexue tongbao,
T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
Recombinant classical swine fever (CSF) viruses derived from the Chinese vaccine strain (C-strain) of CSF virus retain their avirulent and immunogenic characteristics.
May 2000, Vaccine,
T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
Characteristics of virulent, attenuated, and avirulent Mycoplasma pneumoniae strains.
November 1969, Journal of bacteriology,
T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
[In vitro differentiation of avirulent from virulent strains of human Myc. tuberculosis].
March 1968, Igaku to seibutsugaku. Medicine and biology,
T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
Analysis of classical swine fever virus replication kinetics allows differentiation of highly virulent from avirulent strains.
June 2000, Veterinary microbiology,
T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
Monocytosis producing activity from virulent and avirulent strains of Listeria.
January 1990, Acta microbiologica Hungarica,
T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
Serological differentiation of virulent and avirulent strains of Newcastle disease virus.
November 1974, Research in veterinary science,
T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
Genetic relatedness of virulent and avirulent strains of Marek's disease virus.
January 1984, Archives of virology,
T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
Restriction endonuclease analysis of the genomes of virulent and avirulent Marek's disease viruses.
January 1981, Microbiology and immunology,
T Kanno, and T Inoue, and D Mackay, and P Kitching, and S Yamaguchi, and J Shirai
Glycocalyx in virulent and avirulent strains of Shigella flexneri.
July 1988, Journal of clinical pathology,
Copied contents to your clipboard!