Biomaterial Database

Detection of chicken anaemia virus DNA from formalin-fixed tissues by polymerase chain reaction. 1998

K Imai, and M Mase, and S Yamaguchi, and N Yuasa, and K Nakamura

Department of Virology, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.

Chicken anaemia virus (CAV) DNA was detectable from various samples such as cell-free virus, infected MDCC-MSB1 cells, unfixed liver homogenates, formalin-fixed liver homogenate or formalin-fixed paraffin-embedded (FFPE) tissues from experimental or field infected chicks using PCR assay. The detection limit of the first PCR assay was 1 infected cell or 10(-1.5) TCID50 of cell-free virus (strain A2). The nested PCR assay increased the sensitivity 10- or 100-fold. CAV DNA was detectable in the other 14 Japanese strains isolated from 1976 to 1994 by the PCR assay. All the amplified products were digested with BglII, HindIII, PstI and SacI. These results suggest that the region amplified was highly conserved among the strains. The nested PCR assay was very sensitive. However, CAV DNA was detectable in most field samples using the first PCR assay. Therefore, the nested PCR assay may not always be necessary. In contrast, the nested PCR assay was necessary to detect CAV DNA in FFPE tissues or formalin-fixed material. Use of the PCR assay in CAV DNA detection from FFPE tissues may be most valuable in diagnosis of diseases caused by or associated with CAV, because it allows detection of both microscopic lesions and CAV DNA.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D009928	Organ Specificity	Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.	Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D011201	Poultry Diseases	Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild.	Disease, Poultry,Diseases, Poultry,Poultry Disease
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D004279	DNA, Viral	Deoxyribonucleic acid that makes up the genetic material of viruses.	Viral DNA
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D017727	Chicken anemia virus	The type species of GYROVIRUS, a small, non-enveloped DNA virus originally isolated from contaminated vaccines in Japan. It causes chicken infectious anemia and may possibly play a key role in hemorrhagic anemia syndrome, anemia dermatitis, and blue wing disease.	Chicken Anemia Agent,Chicken Infectious Anemia Virus,Infectious Anemia Virus, Chicken,Chicken Anemia Agents,Chicken anemia viruses
D017931	DNA Primers	Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.	DNA Primer,Oligodeoxyribonucleotide Primer,Oligodeoxyribonucleotide Primers,Oligonucleotide Primer,Oligonucleotide Primers,Primer, DNA,Primer, Oligodeoxyribonucleotide,Primer, Oligonucleotide,Primers, DNA,Primers, Oligodeoxyribonucleotide,Primers, Oligonucleotide
D018173	Circoviridae Infections	Virus diseases caused by the CIRCOVIRIDAE.	Circovirus Infections,Infections, Circoviridae,Infections, Circovirus,Circoviridae Infection,Circovirus Infection,Infection, Circoviridae,Infection, Circovirus