Biomaterial Database

Concentration and purification of beta-glucosidase from Aspergillus niger by using aqueous two-phase partitioning. 1998

G Johansson, and K Reczey

Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Sweden. Gote.Johansson@biokem.lu.se

The extracellular enzyme beta-glucosidase, present in a culture filtrate and produced by Aspergillus niger, was concentrated up to 700 times by two-phase partitioning. The two-phase systems were achieved by dissolving dextran and poly(ethylene glycol) in the culture filtrate in such proportions that the lower phase, containing the enzyme, consisted of a very small volume compared with the upper phase. The enzyme had high affinity for the lower phase when the system contained 100 mM KSCN at pH 8.0, and the recoveries of beta-glucosidase were in the range of 85 95% with a concentrating factor of 60-720 times. At the same time, the enzyme was purified 2-3 times.

UI	MeSH Term	Description	Entries
D002623	Chemistry Techniques, Analytical	Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.	Analytical Chemistry Techniques,Analytical Chemistry Methods,Analytical Chemistry Method,Analytical Chemistry Technique,Chemistry Method, Analytical,Chemistry Methods, Analytical,Chemistry Technique, Analytical,Method, Analytical Chemistry,Methods, Analytical Chemistry,Technique, Analytical Chemistry,Techniques, Analytical Chemistry
D005069	Evaluation Studies as Topic	Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies.	Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D001234	Aspergillus niger	An imperfect fungus causing smut or black mold of several fruits and vegetables such as grapes, apricots, onions, and peanuts, and is a common contaminant of food.	Aspergillus lacticoffeatus
D001617	beta-Glucosidase	An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.	Cellobiases,Amygdalase,Cellobiase,Emulsion beta-D-Glucosidase,Gentiobiase,Emulsion beta D Glucosidase,beta Glucosidase,beta-D-Glucosidase, Emulsion
D012492	Salts	Substances produced from the reaction between acids and bases; compounds consisting of a metal (positive) and nonmetal (negative) radical. (Grant & Hackh's Chemical Dictionary, 5th ed)	Salt
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor