BIOMAT Database Logo BIOMAT Database Logo

Impaired hypoxic vasoconstriction in intraacinar microvasculature in hyperoxia-exposed rat lungs. 1998

K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Department of Medicine, School of Medicine, Keio University, Tokyo, Japan.

To assess the effects of exposure of the lung to hyperoxic conditions on reactivity of pulmonary microcirculation to hypoxic stimulation, we measured hypoxia-elicited overall pulmonary pressor changes (HPV) and microvascular diameter changes in intraacinar arterioles, venules, and capillaries in isolated perfused rat lungs exposed to a hyperoxic environment (90% O2). To estimate the importance of vasoactive prostaglandins and nitric oxide (NO) for HPV modification, we examined the roles of constitutive and inducible forms of cyclooxygenase (COX-1 and COX-2) and those of NO synthase (eNOS and iNOS). Indomethacin was used for inhibiting both COX-1 and COX-2, while NS-398 was used as a selective inhibitor of COX-2. Both eNOS and iNOS were suppressed by L-NAME, whereas iNOS alone was inhibited by aminoguanidine. Microvascular diameter was measured with a real-time confocal laser scanning luminescence microscope. We found that (1) exposure to hyperoxia caused overall HPV and arteriolar constriction to be attenuated; (2) the blunted HPV was restored by L-NAME but not by aminoguanidine, indomethacin, or NS-398; and (3) arteriolar constriction was improved by either L-NAME, aminoguanidine, or indomethacin but only slightly by NS-398. In conclusion, attenuation of overall HPV in hyperoxia-exposed lungs is explicable mainly by excessive NO generated via eNOS, while impaired arteriolar constriction is caused by NO yielded by eNOS and iNOS as well as by vasodilating prostaglandin(s) produced by COX-1.

UI MeSH Term Description Entries
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D008297 Male Males
D008833 Microcirculation The circulation of the BLOOD through the MICROVASCULAR NETWORK. Microvascular Blood Flow,Microvascular Circulation,Blood Flow, Microvascular,Circulation, Microvascular,Flow, Microvascular Blood,Microvascular Blood Flows,Microvascular Circulations
D011451 Prostaglandin-Endoperoxide Synthases Enzyme complexes that catalyze the formation of PROSTAGLANDINS from the appropriate unsaturated FATTY ACIDS, molecular OXYGEN, and a reduced acceptor. Fatty Acid Cyclo-Oxygenase,PGH Synthase,Prostaglandin H Synthase,Prostaglandin Synthase,Prostaglandin-Endoperoxide Synthase,Arachidonic Acid Cyclooxygenase,Cyclo-Oxygenase,Cyclooxygenase,Cyclooxygenases,Hydroperoxide Cyclase,PGH2 Synthetase,Prostaglandin Cyclo-Oxygenase,Prostaglandin Cyclooxygenase,Prostaglandin Endoperoxide Synthetase,Prostaglandin G-H Synthase,Prostaglandin H2 Synthetase,Prostaglandin Synthetase,Cyclase, Hydroperoxide,Cyclo Oxygenase,Cyclo-Oxygenase, Fatty Acid,Cyclo-Oxygenase, Prostaglandin,Cyclooxygenase, Arachidonic Acid,Cyclooxygenase, Prostaglandin,Endoperoxide Synthetase, Prostaglandin,Fatty Acid Cyclo Oxygenase,G-H Synthase, Prostaglandin,Prostaglandin Cyclo Oxygenase,Prostaglandin Endoperoxide Synthases,Prostaglandin G H Synthase,Synthase, PGH,Synthase, Prostaglandin,Synthase, Prostaglandin G-H,Synthase, Prostaglandin H,Synthase, Prostaglandin-Endoperoxide,Synthases, Prostaglandin-Endoperoxide,Synthetase, PGH2,Synthetase, Prostaglandin,Synthetase, Prostaglandin Endoperoxide,Synthetase, Prostaglandin H2
D011651 Pulmonary Artery The short wide vessel arising from the conus arteriosus of the right ventricle and conveying unaerated blood to the lungs. Arteries, Pulmonary,Artery, Pulmonary,Pulmonary Arteries
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014661 Vasoconstriction The physiological narrowing of BLOOD VESSELS by contraction of the VASCULAR SMOOTH MUSCLE. Vasoconstrictions
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D052247 Nitric Oxide Synthase Type II A CALCIUM-independent subtype of nitric oxide synthase that may play a role in immune function. It is an inducible enzyme whose expression is transcriptionally regulated by a variety of CYTOKINES. INOS Enzyme,Inducible NOS Protein,Inducible Nitric Oxide Synthase,NOS-II,Nitric Oxide Synthase II,Nitric Oxide Synthase, Type II,NOS II

Related Publications

K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Almitrine mimics hypoxic vasoconstriction in isolated rat lungs.
August 1992, The American journal of physiology,
K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Tolbutamide reverses hypoxic pulmonary vasoconstriction in isolated rat lungs.
November 1989, Quarterly journal of experimental physiology (Cambridge, England),
K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Inhibition of glycolysis potentiates hypoxic vasoconstriction in rat lungs.
November 1983, Journal of applied physiology: respiratory, environmental and exercise physiology,
K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Endotoxin alters hypoxic pulmonary vasoconstriction in isolated rat lungs.
September 1996, Journal of applied physiology (Bethesda, Md. : 1985),
K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Effects of intracellular pH on hypoxic vasoconstriction in rat lungs.
December 1987, Journal of applied physiology (Bethesda, Md. : 1985),
K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Acute pneumonia reversibly inhibits hypoxic vasoconstriction in isolated rat lungs.
January 1992, Physiological research,
K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Almitrine bismesylate reduces hypoxic pulmonary vasoconstriction in isolated rat lungs.
February 1989, The Tohoku journal of experimental medicine,
K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Dexamethasone potentiates hypoxic vasoconstriction in salt solution-perfused rat lungs.
September 1987, The American journal of physiology,
K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Glucose metabolism accelerates the decline of hypoxic vasoconstriction in rat lungs.
May 1981, Respiration physiology,
K Suzuki, and K Naoki, and H Kudo, and K Nishio, and N Sato, and T Aoki, and Y Suzuki, and K Takeshita, and A Miyata, and H Tsumura, and Y Yamakawa, and K Yamaguchi
Leukotriene C4 production during hypoxic pulmonary vasoconstriction in isolated rat lungs.
December 1984, Prostaglandins,
Copied contents to your clipboard!