BIOMAT Database Logo BIOMAT Database Logo

Effect of DNA-interacting drugs on phage T7 RNA polymerase. 1998

M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
Department of General Chemistry, Institute of Physiology and Biochemistry, Medical University in Lódz, Poland.

9-Aminoacridine carboxamide derivatives studied here form with DNA intercalative complexes which differ in the kinetics of dissociation. Inhibition of total RNA synthesis catalyzed by phage T7 and Escherichia coli DNA-dependent RNA polymerases correlates with the formation of slowly dissociating acridine-DNA complex of time constant of 0.4-2.3 s. Their effect on RNA synthesis is compared with other ligands which form with DNA stable complexes of different steric properties. T7 RNA polymerase is more sensitive to distamycin A and netropsin than the E. coli enzyme while less sensitive to actinomycin D. Actinomycin induces terminations in the transcript synthesized by T7 RNA polymerase. Despite low dissociation rates of DNA complexes with acridines and pyrrole antibiotics no drug dependent terminations are observed with these ligands.

UI MeSH Term Description Entries
D007364 Intercalating Agents Agents that are capable of inserting themselves between the successive bases in DNA, thus kinking, uncoiling or otherwise deforming it and therefore preventing its proper functioning. They are used in the study of DNA. Intercalating Agent,Intercalating Ligand,Intercalative Compound,Intercalator,Intercalators,Intercalating Ligands,Intercalative Compounds,Agent, Intercalating,Agents, Intercalating,Compound, Intercalative,Compounds, Intercalative,Ligand, Intercalating,Ligands, Intercalating
D009429 Netropsin A basic polypeptide isolated from Streptomyces netropsis. It is cytotoxic and its strong, specific binding to A-T areas of DNA is useful to genetics research. Sinanomycin,Congocidine,IA-887,Netropsin Hydrochloride,T-1384,Hydrochloride, Netropsin
D003609 Dactinomycin A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015) Actinomycin,Actinomycin D,Meractinomycin,Cosmegen,Cosmegen Lyovac,Lyovac-Cosmegen,Lyovac Cosmegen,Lyovac, Cosmegen,LyovacCosmegen
D004214 Distamycins Oligopeptide antibiotics from Streptomyces distallicus. Their binding to DNA inhibits synthesis of nucleic acids.
D000577 Amides Organic compounds containing the -CO-NH2 radical. Amides are derived from acids by replacement of -OH by -NH2 or from ammonia by the replacement of H by an acyl group. (From Grant & Hackh's Chemical Dictionary, 5th ed) Amide
D000609 Aminoacridines Acridines which are substituted in any position by one or more amino groups or substituted amino groups.
D000900 Anti-Bacterial Agents Substances that inhibit the growth or reproduction of BACTERIA. Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D012321 DNA-Directed RNA Polymerases Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992). DNA-Dependent RNA Polymerases,RNA Polymerases,Transcriptases,DNA-Directed RNA Polymerase,RNA Polymerase,Transcriptase,DNA Dependent RNA Polymerases,DNA Directed RNA Polymerase,DNA Directed RNA Polymerases,Polymerase, DNA-Directed RNA,Polymerase, RNA,Polymerases, DNA-Dependent RNA,Polymerases, DNA-Directed RNA,Polymerases, RNA,RNA Polymerase, DNA-Directed,RNA Polymerases, DNA-Dependent,RNA Polymerases, DNA-Directed
D017123 Bacteriophage T7 Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted. Coliphage T7,Enterobacteria phage T7,Phage T7,T7 Phage,Phage, T7,Phages, T7,T7 Phages

Related Publications

M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
Replication of RNA by the DNA-dependent RNA polymerase of phage T7.
May 1989, Cell,
M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
RNA polymerase synthesis in vitro directed by T7 phage DNA.
January 1971, Molecular & general genetics : MGG,
M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
Transcription of late phage RNA by T7 RNA polymerase.
December 1970, Nature,
M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
Effect of salt on the transcription of T7 DNA by RNA polymerase from T4 phage-infected E.coli.
April 1977, Nucleic acids research,
M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
Initiation by RNA polymerase on alkylated T7 DNA.
August 1972, Biochimica et biophysica acta,
M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
[RNA synthesis using T7 phage RNA polymerase: transcription of synthetic DNA templates in solution and on polymer support].
February 1989, Bioorganicheskaia khimiia,
M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
[Effect of the structure of the prepromotor region on transcription using phage T7 RNA polymerase].
January 1996, Molekuliarnaia biologiia,
M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
[Affinity modification of DNA-dependent RNA-polymerase of phage T7 with 5'-p-fluorosulfonylbenzoyladenosine].
January 1988, Molekuliarnaia biologiia,
M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
Effect of rifampicin on DNA-dependent RNA polymerase and on RNA phage growth.
January 1968, Cold Spring Harbor symposia on quantitative biology,
M Piestrzeniewicz, and K Studzian, and D Wilmańska, and G Płucienniczak, and M Gniazdowski
[Phage T7 RNA-polymerase: gene cloning and its structure].
June 1984, Bioorganicheskaia khimiia,
Copied contents to your clipboard!