BIOMAT Database Logo BIOMAT Database Logo

Function of hexameric RNA in packaging of bacteriophage phi 29 DNA in vitro. 1998

F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
Department of Genetics and Cell Biology, University of Minnesota, Minneapolis 55455, USA.

A cyclic hexamer of the 120-base prohead RNA (pRNA) is needed for efficient in vitro packaging of the B. subtilis bacteriophage phi 29 genome. This capacity of pRNA to form higher multimers by intermolecular base pairing of identical subunits represents a new RNA structural motif. Dimers of pRNA are likely intermediates in formation of the cyclic hexamer. A three-dimensional model of the pRNA hexamer is presented.

UI MeSH Term Description Entries
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009024 Morphogenesis The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D011108 Polymers Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS). Polymer
D002213 Capsid The outer protein protective shell of a virus, which protects the viral nucleic acid. Capsids are composed of repeating units (capsomers or capsomeres) of CAPSID PROTEINS which when assembled together form either an icosahedral or helical shape. Procapsid,Prohead,Capsids,Procapsids,Proheads
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D000079962 RNA, Circular RNA molecules in which the 3' and 5' ends are covalently joined to form a closed continuous loop. They are resistant to digestion by EXORIBONUCLEASES. Circular Intronic RNA,Circular RNA,Circular RNAs,Closed Circular RNA,ciRNA,circRNA,circRNAs,Circular RNA, Closed,Intronic RNA, Circular,RNA, Circular Intronic,RNA, Closed Circular,RNAs, Circular
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated

Related Publications

F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
Role of RNA in bacteriophage phi 29 DNA packaging.
January 1990, Journal of structural biology,
F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
Bacteriophage phi 29 DNA packaging.
January 2002, Advances in virus research,
F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
RNA dependence of the bacteriophage phi 29 DNA packaging ATPase.
October 1990, Journal of molecular biology,
F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
Identification of bacteriophage phi 29 prohead RNA domains necessary for in vitro DNA-gp3 packaging.
March 1994, The Journal of biological chemistry,
F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
A small viral RNA is required for in vitro packaging of bacteriophage phi 29 DNA.
May 1987, Science (New York, N.Y.),
F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
Bacteriophage phi 29 proteins required for in vitro DNA-gp3 packaging.
June 1984, Journal of virology,
F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
Initiation events in in-vitro packaging of bacteriophage phi 29 DNA-gp3.
September 1987, Journal of molecular biology,
F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
Characterization of the small RNA of the bacteriophage phi 29 DNA packaging machine.
September 1987, Nucleic acids research,
F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
Mutant prohead RNAs in the in vitro packaging of bacteriophage phi 29 DNA-gp3.
February 1992, Journal of molecular biology,
F Zhang, and S Lemieux, and X Wu, and D St-Arnaud, and C T McMurray, and F Major, and D Anderson
In vitro replication of bacteriophage phi 29 DNA.
September 1982, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!