RLU and studies using the luciferase reporter gene.
1998
J M Vicat, and
D Blum, and
D Wion, and
A L Benabid, and
F Berger
UI
MeSH Term
Description
Entries
D008156
Luciferases
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
Luciferase
D002138
Calibration
Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.
Calibrations
D014162
Transfection
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Transfections
D049454
Firefly Luciferin
A benzothaizole which is oxidized by LUCIFERASES, FIREFLY to cause emission of light (LUMINESCENCE).
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Reporter Genes,Gene, Reporter,Reporter Gene
Related Publications
J M Vicat, and
D Blum, and
D Wion, and
A L Benabid, and
F Berger