Elevated hypoxanthine (Hx) levels in the vitreous humor of victims of the sudden infant death syndrome (SIDS) have been been claimed to indicate hypoxia before death. An important methodologic problem is the temperature-dependent Hx increase after death. Although most previous studies have corrected for expected postmortem Hx increase, the aim of the present study was to omit this problem by matching the subjects of the different groups studied for similar postmortem time. Thirteen cases of violent death, 11 cases of borderline SIDS, 22 cases of death from infectious disease, and 17 cases of death from heart/lung disease were compared with a 3-fold number of SIDS cases. To investigate the impact of environmental factors on the Hx levels of the SIDS cases, the following possible confounding factors were investigated: attempt to resuscitate, slight infections before death, sleeping position, and time of the day when found dead. To investigate whether the size of the eyeball would influence the Hx level, five cases of violent death in children were compared with 15 adults who died suddenly. The vitreous humor Hx level was significantly higher in SIDS victims than in cases of violent death (p < 0.01) and deaths due to heart/lung disease (p < 0.01), whereas no such difference was found either between SIDS and borderline SIDS or SIDS and infectious death. There were no differences between any of the subgroups of SIDS victims. The vitreous humor Hx level in small children and adults did not differ. The findings raise the question of possible similarities in death mechanism in SIDS and infectious death.