Assessment of autosome and gonosome disomy in human sperm nuclei by chromosome painting. 1998

N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
Laboratoire de Cytogénétique-Biologie de la Reproduction, Centre Hospitalier Universitaire Charles Nicolle, Rouen, France. Bertrand.Mace@chu-Rouen.fr

Disomy and diploidy frequencies for autosomes 1-22 and the gonosomes were assessed in 299,442 sperm nuclei from four normal fertile men by chromosome painting. This novel approach allowed us to perform a specific and sensitive detection of each chromosome. A minimum of 5000 sperm nuclei per subject were evaluated for each chromosome by dual colour fluorescence in situ hybridization. The disomy rate proved to be similar for all the autosomes (0.24%) and the diploidy rate varied from 0.12% to 0.15%. No interchromosomal or interindividual differences in the frequency of disomic and diploid sperm nuclei were observed between the four subjects. The mean frequency of XX-, YY- and XY-bearing spermatozoa was estimated to 0.17%, 0.17% and 0.32%, respectively. This strategy constitutes a new approach for detecting aneuploidy in human sperm nuclei and suggests an equal repartition of non-disjunction among chromosomes in male gametes.

UI MeSH Term Description Entries
D008297 Male Males
D009630 Nondisjunction, Genetic The failure of homologous CHROMOSOMES or CHROMATIDS to segregate during MITOSIS or MEIOSIS with the result that one daughter cell has both of a pair of parental chromosomes or chromatids and the other has none. Genetic Non-Disjunction,Genetic Nondisjunction,Non-Disjunction, Genetic,Genetic Non Disjunction,Genetic Non-Disjunctions,Genetic Nondisjunctions,Non Disjunction, Genetic,Non-Disjunctions, Genetic,Nondisjunctions, Genetic
D004171 Diploidy The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X. Diploid,Diploid Cell,Cell, Diploid,Cells, Diploid,Diploid Cells,Diploidies,Diploids
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000782 Aneuploidy The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1). Aneuploid,Aneuploid Cell,Aneuploid Cells,Aneuploidies,Aneuploids,Cell, Aneuploid,Cells, Aneuploid
D012730 Sex Chromosomes The homologous chromosomes that are dissimilar in the heterogametic sex. There are the X CHROMOSOME, the Y CHROMOSOME, and the W, Z chromosomes (in animals in which the female is the heterogametic sex (the silkworm moth Bombyx mori, for example)). In such cases the W chromosome is the female-determining and the male is ZZ. (From King & Stansfield, A Dictionary of Genetics, 4th ed) Gonosomes,Chromosome, Sex,Chromosomes, Sex,Gonosome,Sex Chromosome
D013094 Spermatozoa Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility. Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms
D017404 In Situ Hybridization, Fluorescence A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei. FISH Technique,Fluorescent in Situ Hybridization,Hybridization in Situ, Fluorescence,FISH Technic,Hybridization in Situ, Fluorescent,In Situ Hybridization, Fluorescent,FISH Technics,FISH Techniques,Technic, FISH,Technics, FISH,Technique, FISH,Techniques, FISH

Related Publications

N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
April 1994, Human genetics,
N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
September 1967, Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie,
N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
December 2002, American journal of human genetics,
N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
October 2014, Human reproduction (Oxford, England),
N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
January 1992, European journal of histochemistry : EJH,
N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
January 1994, Human genetics,
N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
January 1998, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology,
N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
October 2017, International journal of environmental health research,
N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
January 1998, Cytogenetics and cell genetics,
N Rives, and S Mazurier, and D Bellet, and G Joly, and B Macé
January 1997, Cytogenetics and cell genetics,
Copied contents to your clipboard!